Genome-wide identification, phylogenetic analysis, expression profiling, and protein-protein interaction properties of TOPLESS gene family members in tomato.
Genome-wide identification, phylogenetic analysis, expression profiling, and protein-protein interaction properties of TOPLESS gene family members in tomato.
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DOI：
10.1093/jxb/ert440
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发表时间：
2014-03
影响因子：
6.9
通讯作者：
Zouine M
中科院分区：
文献类型：
--
作者：
Hao Y;Wang X;Li X;Bassa C;Mila I;Audran C;Maza E;Li Z;Bouzayen M;van der Rest B;Zouine M
裸照家族的成员最近在几种机制中成为基因表达的主要参与者，尤其是裸露的基因。 （SLTPL）在番茄基因组中鉴定出含有规范的域（LISH，CTLH和两个WD40重复量）。 UES除了成熟的水果外，SLTPL3和SLTPL4的成熟是最突出的，以了解不同裸照的特异性，一种蛋白质 - 蛋白质的相互作用图是使用YEAST ISSTORMERERS构建的，这是两种模式。 AUX/IAA和同工型有趣的是，与这些蛋白质伴侣的相互作用有限。
Members of the TOPLESS gene family emerged recently as key players in gene repression in several mechanisms, especially in auxin perception. The TOPLESS genes constitute, in ‘higher-plant’ genomes, a small multigenic family comprising four to 11 members. In this study, this family was investigated in tomato, a model plant for Solanaceae species and fleshy fruits. Six open reading frames predicted to encode topless-like proteins (SlTPLs) containing the canonical domains (LisH, CTLH, and two WD40 repeats) were identified in the tomato genome. Nuclear localization was confirmed for all members of the SlTPL family with the exception SlTPL6, which localized at the cytoplasm and was excluded from the nucleus. SlTPL genes displayed distinctive expression patterns in different tomato organs, with SlTPL1 showing the highest levels of transcript accumulation in all tissues tested except in ripening fruit where SlTPL3 and SlTPL4 were the most prominently expressed. To gain insight into the specificity of the different TOPLESS paralogues, a protein–protein interaction map between TOPLESS and auxin/indole-3-acetic acid (Aux/IAA) proteins was built using a yeast two-hybrid approach. The PPI map enabled the distinction of two patterns: TOPLESS isoforms interacting with the majority of Aux/IAA, and isoforms with limited capacity for interaction with these protein partners. Interestingly, evolutionary analyses of the TOPLESS gene family revealed that the highly expressed isoforms (SlTPL1, SlTPL3, and SlTPL4) corresponded to the three TPL-related genes undergoing the strongest purifying selection, while the selection was much weaker for SlTPL6, which was expressed at a low level and encoded a protein lacking the capacity to interact with Aux/IAAs.
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