Guidelines for the use of cell lines in biomedical research.
Guidelines for the use of cell lines in biomedical research.
复制标题
DOI：
10.1038/bjc.2014.166
复制
发表时间：
2014-09-09
影响因子：
8.8
通讯作者：
Vias, M.
中科院分区：
文献类型：
--
作者：
Geraghty, R. J.;Capes-Davis, A.;Davis, J. M.;Downward, J.;Freshney, R. I.;Knezevic, I.;Lovell-Badge, R.;Masters, J. R. W.;Meredith, J.;Stacey, G. N.;Thraves, P.;Vias, M.
关键词：
细胞系错误鉴定和微生物（如支原体）污染以及遗传和表型不稳定性是继续影响细胞培养的问题之一。如果有必要的远见，这些问题中有许多是可以避免的，编写这些指导方针是为了向那些新进入这一领域的人和其他从事教学和指导的人提供必要的信息，以提高他们对这些问题的认识，使他们能够有效地处理这些问题。该指南涵盖了开发、获取、认证、冷冻保存、实验室间细胞系转移、微生物污染、表征、不稳定性和错误鉴定等领域。此外，还就从人类和动物组织中提取细胞系时遵守现行法律的和伦理要求、设备的选择和维护以及如何处理可能出现的问题提供了建议。
Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.
登录
查看更多内容
影响因子：
8.5
作者：
Choumerianou, D. M.;Dimitriou, H.;Kalmanti, M.
通讯作者：
Kalmanti, M.
影响因子：
2.2
作者：
Bandi, Sriram;Akkina, Ramesh
通讯作者：
Akkina, Ramesh
影响因子：
11.2
作者：
Bernardo, Maria Ester;Zaffaroni, Nadia;Locatelli, Franco
通讯作者：
Locatelli, Franco
影响因子：
2.2
作者：
Freshney, RI
通讯作者：
Freshney, RI
影响因子：
2.7
作者：
Coecke, S;Balls, M;Stokes, W
通讯作者：
Stokes, W