Aberrant spliceosome expression and altered alternative splicing events correlate with maturation deficiency in human oocytes.
Aberrant spliceosome expression and altered alternative splicing events correlate with maturation deficiency in human oocytes.
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异常剪接体表达和改变的选择性剪接事件与人类卵母细胞成熟缺陷相关
DOI：
10.1080/15384101.2020.1799295
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Li J;Lu M;Zhang P;Hou E;Li T;Liu X;Xu X;Wang Z;Fan Y;Zhen X;Li R;Liu P;Yu Y;Hang J;Qiao J
卵巢刺激的不同策略在IVF中被广泛用于检索成熟的中期II（MII）卵母细胞的施肥，大约有70％的卵母细胞成熟，而个性化的卵母细胞是成熟的IVM的管理可以进一步提高成熟率，即使在这样的条件下，仍然没有偶然地观察到卵母细胞的成熟。在本研究中，这种成熟缺乏（MD）卵母细胞在中期I（MI）阶段的绝对造成。与正常成熟的（MN）卵母细胞相比，MD卵母细胞中的替代剪接（AS）模式是异常的。不同表达的基因（DEG）主要与MRNA剪接，RNA转运，RNA加工和mRNA调节相关，随后，AS的分析显示，AS AS的基因主要与代谢和细胞周期有关这些发现，我们在完全成熟的缺乏卵母细胞中证明了异常基因表达，我们提出了转录后调节的改变构成了控制卵母细胞成熟的潜在潜在机制。
ABSTRACT Different strategies of ovarian stimulation are widely used in IVF to retrieve mature metaphase II (MII) oocytes for fertilization. On average, approximately 70% of recovered oocytes are mature, while personalized administration of hCG and/or GnRH agonist trigger and in vitro maturation (IVM) management can further improve the maturation rate. However, even under such conditions, a complete absence of oocyte maturation is still observed sporadically. The probable causes for such maturation-deficient (MD) oocytes – which arrest abnormally at metaphase I (MI) stage – are still under investigation. In the present study, using single-cell transcriptomic RNA sequencing (RNA-seq) and differential expression analysis, we showed that gene expression profiles were aberrant, and alternative splicing (AS) patterns were changed in MD oocytes when compared with normally mature (MN) oocytes. Gene ontology (GO) enrichment demonstrated that the differently expressed genes (DEGs) were mostly correlated with pre-mRNA splicing, RNA transportation, RNA processing, and mRNA regulation. Subsequently, analysis of AS events revealed that genes with altered AS patterns were primarily associated with metabolism and cell cycle. With these findings, we have demonstrated aberrant gene expression in complete maturation-deficient oocytes, and we propose that alterations in post-transcriptional regulation constitute a potential underlying mechanism governing oocyte maturation.
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