Phenotypic variation and host interactions of Xenorhabdus bovienii SS-2004, the entomopathogenic symbiont of Steinernema jollieti nematodes.
Phenotypic variation and host interactions of Xenorhabdus bovienii SS-2004, the entomopathogenic symbiont of Steinernema jollieti nematodes.
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DOI：
10.1111/j.1462-2920.2011.02663.x
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发表时间：
2012-04
影响因子：
5.1
通讯作者：
Goodrich-Blair H
中科院分区：
文献类型：
--
作者：
Sugar DR;Murfin KE;Chaston JM;Andersen AW;Richards GR;deLéon L;Baum JA;Clinton WP;Forst S;Goldman BS;Krasomil-Osterfeld KC;Slater S;Stock SP;Goodrich-Blair H
博氏短杆菌（SS-2004）细菌存在于昆虫病原线虫（Steinernema jollieti）的感染-幼年（IJ）期的肠道中。最近的X. bovienii基因组促进其作为理解宿主-共生体相互作用的模型的使用。为了给这些研究提供生物学基础，我们对X. bovienii在体外和宿主相互作用表型。在线虫寄主X. Bovienii被包含在膜结合的包膜内，该包膜也封闭了线虫衍生的囊内结构。S.在X.表达绿色或DsRed荧光蛋白的bovienii主要被一种或另一种菌株定殖，表明定殖群体由少数细胞建立。X. bovienii在橙色初级型和奶油色次级型之间表现出表型变异。每一种形式都可以定殖IJ线虫时，在体外培养的琼脂。然而，IJs并没有发展或出现从大蜡螟昆虫感染的第二形式。与柔软灵活的初级形式感染昆虫不同，次级形式感染昆虫保留了坚硬的外骨骼结构。X. bovienii初级和次级形式分离物对烟草天蛾和几种其它昆虫具有毒性。然而，初级形式股票呈现减弱的毒力，这表明X。bovienii和X.嗜水气单胞菌可能经历毒力调节。
Xenorhabdus bovienii (SS-2004) bacteria reside in the intestine of the infective-juvenile (IJ) stage of the entomopathogenic nematode, Steinernema jollieti. The recent sequencing of the X. bovienii genome facilitates its use as a model to understand host-symbiont interactions. To provide a biological foundation for such studies, we characterized X. bovienii in vitro and host-interaction phenotypes. Within the nematode host X. bovienii was contained within a membrane bound envelope that also enclosed the nematode-derived intravesicular structure. S. jollieti nematodes cultivated on mixed lawns of X. bovienii expressing green or DsRed fluorescent proteins were predominantly colonized by one or the other strain, suggesting the colonizing population is founded by a few cells. X. bovienii exhibits phenotypic variation between orange-pigmented primary form and cream-pigmented secondary form. Each form can colonize IJ nematodes when cultured in vitro on agar. However, IJs did not develop or emerge from Galleria mellonella insects infected with secondary form. Unlike primary-form infected insects that were soft and flexible, secondary-form infected insects retained a rigid exoskeleton structure. X. bovienii primary and secondary form isolates are virulent toward Manduca sexta and several other insects. However, primary form stocks present attenuated virulence, suggesting that X. bovienii, like X. nematophila may undergo virulence modulation.
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DOI：
10.1073/pnas.102068099
发表时间：
2002-08-06
影响因子：
11.1
作者：
Daborn, PJ;Waterfield, N;Ffrench-Constant, RH
通讯作者：
Ffrench-Constant, RH
影响因子：
3.4
作者：
Emelianoff, Vanya;Le Brun, Nathalie;Sicard, Mathieu
通讯作者：
Sicard, Mathieu
影响因子：
4
作者：
BIRD, AF;AKHURST, RJ
通讯作者：
AKHURST, RJ
影响因子：
3.2
作者：
Cowles, Charles E.;Goodrich-Blair, Heidi
通讯作者：
Goodrich-Blair, Heidi
影响因子：
3.4
作者：
Campos-Herrera, R.;Tailliez, P.;Boemare, N. E.
通讯作者：
Boemare, N. E.