Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness.
Actin filament reorganisation controlled by the SCAR/WAVE complex mediates stomatal response to darkness.
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由 SCAR/WAVE 复合物控制的肌动蛋白丝重组介导气孔对黑暗的反应
DOI：
10.1111/nph.14655
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Hetherington AM
中科院分区：
文献类型：
--
作者：
Isner JC;Xu Z;Costa JM;Monnet F;Batstone T;Ou X;Deeks MJ;Genty B;Jiang K;Hetherington AM
气孔对黑暗的反应是关闭，以防止夜间过度失水。虽然气孔关闭过程中肌动蛋白丝的重组被记录，但暗诱导细胞骨架排列的潜在机制仍然很大程度上未知。我们使用遗传学，生理学和细胞生物学的方法来表明，肌动蛋白细胞骨架的重组是暗诱导气孔关闭所必需的。opal 5突变体不会响应黑暗而关闭，但当暴露于脱落酸（阿坝）或CaCl 2时表现出野生型（WT）行为。该突变定位于At 5g 18410，编码拟南芥SCAR/WAVE复合物的PIR/SRA 1/KLK亚基。PIR基因的一个独立等位基因（Atpir-1）的气孔显示出对黑暗的敏感性降低，opal 5和Atpir-1杂交的F1后代显示出扭曲的叶毛状体，表明这两个突变体是等位的。黑暗诱导WT中肌动蛋白丝成束程度的变化。这些在opal 5中被废除了。使用latrunculin B或细胞松弛素D破坏丝状肌动蛋白恢复了opal 5中野生型气孔对黑暗的敏感性。我们的研究结果表明，气孔对黑暗的反应是介导的重组保卫细胞肌动蛋白丝，一个过程，是精心调整的保守的SCAR/WAVE-Arp 2/3肌动蛋白调控模块。
Stomata respond to darkness by closing to prevent excessive water loss during the night. Although the reorganisation of actin filaments during stomatal closure is documented, the underlying mechanisms responsible for dark‐induced cytoskeletal arrangement remain largely unknown. We used genetic, physiological and cell biological approaches to show that reorganisation of the actin cytoskeleton is required for dark‐induced stomatal closure. The opal5 mutant does not close in response to darkness but exhibits wild‐type (WT) behaviour when exposed to abscisic acid (ABA) or CaCl2. The mutation was mapped to At5g18410, encoding the PIR/SRA1/KLK subunit of the Arabidopsis SCAR/WAVE complex. Stomata of an independent allele of the PIR gene (Atpir‐1) showed reduced sensitivity to darkness and F1 progenies of the cross between opal5 and Atpir‐1 displayed distorted leaf trichomes, suggesting that the two mutants are allelic. Darkness induced changes in the extent of actin filament bundling in WT. These were abolished in opal5. Disruption of filamentous actin using latrunculin B or cytochalasin D restored wild‐type stomatal sensitivity to darkness in opal5. Our findings suggest that the stomatal response to darkness is mediated by reorganisation of guard cell actin filaments, a process that is finely tuned by the conserved SCAR/WAVE–Arp2/3 actin regulatory module.
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