Factors modulating expression of Renilla luciferase from control plasmids used in luciferase reporter gene assays.
Factors modulating expression of Renilla luciferase from control plasmids used in luciferase reporter gene assays.
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DOI：
10.1016/j.ab.2009.09.043
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发表时间：
2010-01-15
影响因子：
2.9
通讯作者：
Hardin JA
中科院分区：
文献类型：
--
作者：
Shifera AS;Hardin JA
海肾荧光素酶基因通常用作基于内切酶的报告基因测定中的内部对照，以针对可能由转染效率和样品处理引起的变化将实验报告基因的值标准化。在不同启动子构建体下编码海肾荧光素酶的各种质粒是可商购的。使用海肾荧光素酶作为内部对照的有效性基于以下假设：其在转染细胞中组成型表达，并且其组成型表达不受可能导致所产生的酶量上调或下调的实验因素调节。在过去的十年中，已经出现了许多报告，确定了各种条件下，可以改变海肾荧光素酶的基础组成型表达。在这些情况下使用海肾荧光素酶将是无效的，并且需要替代的标准化方法。这篇综述涵盖了迄今为止已报道的调节海肾荧光素酶从质粒构建体的表达的因素。
The Renilla luciferase gene is commonly used as an internal control in luciferase-based reporter gene assays to normalize the values of the experimental reporter gene for variations that could be caused by transfection efficiency and sample handling. Various plasmids encoding Renilla luciferase under different promoter constructs are commercially available. The validity of the use of Renilla luciferase as an internal control is based on the assumption that it is constitutively expressed in transfected cells and that its constitutive expression is not modulated by experimental factors that could result in either the upregulation or the downregulation of the amounts of the enzyme produced. During the past ten years, a number of reports have appeared that identified a variety of conditions that could alter the basal constitutive expression of Renilla luciferase. The use of Renilla luciferase in those circumstances would not be valid and an alternative way of normalization would be necessary. This review covers the factors that have been reported thus far as modulating the expression of Renilla luciferase from plasmid constructs.
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影响因子：
48
作者：
Loening, Andreas Markus;Wu, Anna M.;Gambhir, Sanjiv Sam
通讯作者：
Gambhir, Sanjiv Sam
DOI：
10.1016/s0960-0760(99)00109-0
发表时间：
1999-09-01
影响因子：
4.1
作者：
Everett, LM;Crabb, DW
通讯作者：
Crabb, DW
影响因子：
64.5
作者：
JONES, KA;YAMAMOTO, KR;TJIAN, R
通讯作者：
TJIAN, R
影响因子：
56.9
作者：
COEN, DM;WEINHEIMER, SP;MCKNIGHT, SL
通讯作者：
MCKNIGHT, SL
影响因子：
4.8
作者：
Dittmer, J;PiseMasison, CA;Brady, JN
通讯作者：
Brady, JN