Effect of Electrical Stimulation of Cervical Sympathetic Ganglia on Intraocular Pressure Regulation According to Different Circadian Rhythms in Rats.
Effect of Electrical Stimulation of Cervical Sympathetic Ganglia on Intraocular Pressure Regulation According to Different Circadian Rhythms in Rats.
复制标题
根据不同昼夜节律电刺激颈部交感神经节对大鼠眼压调节的影响。
DOI：
10.1167/iovs.61.11.40
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发表时间：
2020-09-01
影响因子：
4.4
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Luo Z;Li M;Ye M;Ji P;Lou X;Huang J;Yao K;Zhao Y;Zhang H
本研究旨在探究昼夜节律与眼压（IOP）之间的关系，并探讨对大鼠颈交感神经节（SCG）进行电刺激是否能通过施莱姆管（SC）周围的神经递质分布来调节眼压。
将Sprague Dawley大鼠置于正常节律（N - 正常）、持续黑暗（N - 黑暗）和持续光照（N - 光照）环境中饲养（每组n = 6只）。采用电刺激（间歇波[20赫兹（Hz），2毫安，10分钟]）刺激颈交感神经节。每天三次应用硫酸阿托品眼用凝胶。将DiI注入颈交感神经节和前房。使用苏木精 - 伊红染色评估施莱姆管的横截面积和周长。采用免疫荧光染色评估施莱姆管内皮（SCE）细胞中多巴胺-β-羟化酶（DβH）的表达。
N - 黑暗环境升高了眼压，减小了施莱姆管的横截面积，并增加了施莱姆管内皮细胞中的DβH水平。检测到施莱姆管与颈交感神经节之间存在神经投射，对颈交感神经节进行电刺激上调了施莱姆管内皮细胞中DβH的表达。在正常和持续光照节律下，对颈交感神经节进行电刺激增加了DβH表达，减小了施莱姆管的横截面积和周长，同时升高了眼压并降低了眼压波动。在睫状肌麻痹后，在正常和持续光照节律下对颈交感神经节进行电刺激减小了施莱姆管的横截面积和周长。
N - 黑暗环境增加了施莱姆管内皮细胞中的DβH，减小了施莱姆管的横截面积，并升高了眼压。在正常和光照节律下，对颈交感神经节进行电刺激增加了施莱姆管内皮细胞中的DβH，减小了施莱姆管的横截面积和周长，进而升高了眼压并降低了眼压波动。
The purpose of this study was to investigate the relationship between circadian rhythm and intraocular pressure (IOP), and to explore whether electrical stimulation of cervical sympathetic ganglia (SCG) can regulate IOP via neurotransmitter distribution around the Schlemm's canal (SC) in rats. Sprague Dawley rats were housed under normal (N-normal), constant dark (N-dark), and constant light (N-light) rhythms (n = 6 per group). Electrical stimulation (intermittent wave [20 hertz {Hz}, 2 mA, 10 minutes]) was used to stimulate the SCG. Atropine sulfate eye gel was applied three times a day. DiI was injected into the SCG and anterior chamber. The cross-sectional area and circumference of SC were evaluated using hematoxylin-eosin staining. Immunofluorescence staining was used to evaluate dopamine-β-hydroxylase (DβH) expression in SC endothelial (SCE) cells. N-Dark increased the IOP, decreased the cross-sectional area of SC, and increased DβH levels in SCE cells. Nerve projection between SC and SCG was detected, and electrical stimulation of SCG upregulated DβH expression in SCE cells. Under normal and constant light rhythms, electrical stimulation of SCG increased DβH and decreased the cross-sectional area and circumference of SC, while simultaneously increasing IOP and decreasing IOP fluctuations. After paralyzing the ciliary muscles, electrical stimulation of SCG decreased the cross-sectional area and circumference of SC under normal and constant light rhythms. N-Dark increased DβH in SCE cells, reduced the cross-sectional area of SC, and increased IOP. Under the normal and light rhythms, electrical stimulation of SCG increased DβH in SCE cells, reduced the cross-sectional area and circumference of SC, and in turn elevated IOP and decreased IOP fluctuations.
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影响因子：
2
作者：
Hughes, E;Spry, P;Diamond, J
通讯作者：
Diamond, J
影响因子：
3.4
作者：
Ji, Pingting;Chen, Liwen;Zhang, Hong
通讯作者：
Zhang, Hong
DOI：
10.1007/s00417-011-1728-3
发表时间：
2011-10-01
影响因子：
2.7
作者：
Bull, Holger;von Wolff, Kurt;Tetz, Manfred
通讯作者：
Tetz, Manfred
影响因子：
13.7
作者：
Chui, Wan-sang;Lam, Andrew;Chiu, Roger
通讯作者：
Chiu, Roger
影响因子：
13.7
作者：
De Moraes, Carlos Gustavo;Jasien, Jessica V.;Ritch, Robert
通讯作者：
Ritch, Robert