Discovery of unconventional kinetochores in kinetoplastids.
Discovery of unconventional kinetochores in kinetoplastids.
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DOI：
10.1016/j.cell.2014.01.049
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发表时间：
2014-03-13
期刊：
影响因子：
64.5
通讯作者：
Gull K
中科院分区：
文献类型：
--
作者：
Akiyoshi B;Gull K
动粒是真核生物中指导染色体分离的大分子蛋白质复合物。人们普遍认为，核心动粒由所有真核生物共有的蛋白质组成。然而，在任何动质体基因组中都没有发现传统的动粒成分，因此对这种普遍性的假设提出了挑战。在这里，我们报告的19个着丝粒蛋白（KKT 1 -19）的布氏锥虫的鉴定。大多数在动质体中是保守的，但它们中没有一个与常规动粒蛋白具有可检测的同源性。相反，这些蛋白质具有在常规动粒蛋白中未发现的各种特征。我们认为动质体使用一组不同的蛋白质来构建动粒。这些发现为真核生物生命之树的根的位置这个长期存在的问题提供了重要的见解。在任何动质体基因组中都不能鉴定出常规的动粒蛋白，在布氏锥虫中鉴定出19种动粒蛋白。动质体具有非常规的动粒。这一发现支持了动质体很早就分支的假设。在布氏锥虫中鉴定出了一组构成动粒的非常规蛋白，这为动质体疾病提供了一个有吸引力的药物靶标。
The kinetochore is the macromolecular protein complex that directs chromosome segregation in eukaryotes. It has been widely assumed that the core kinetochore consists of proteins that are common to all eukaryotes. However, no conventional kinetochore components have been identified in any kinetoplastid genome, thus challenging this assumption of universality. Here, we report the identification of 19 kinetochore proteins (KKT1–19) in Trypanosoma brucei. The majority is conserved among kinetoplastids, but none of them has detectable homology to conventional kinetochore proteins. These proteins instead have a variety of features not found in conventional kinetochore proteins. We propose that kinetoplastids build kinetochores using a distinct set of proteins. These findings provide important insights into the longstanding problem of the position of the root of the eukaryotic tree of life. Conventional kinetochore proteins cannot be identified in any kinetoplastid genome 19 kinetochore proteins were identified in Trypanosoma brucei Kinetoplastids possess unconventional kinetochores This discovery supports the hypothesis that kinetoplastids branched very early A group of unconventional proteins constituting the kinetochore in Trypanosoma brucei has been identified, which provides an attractive drug target for kinetoplastid diseases.
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