Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate.
Functional and histologic imaging of urinary bladder wall after exposure to psychological stress and protamine sulfate.
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DOI：
10.1038/s41598-021-98504-9
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发表时间：
2021-09-30
影响因子：
4.6
通讯作者：
Tyagi P
中科院分区：
文献类型：
--
作者：
Saito T;Hitchens TK;Foley LM;Singh N;Mizoguchi S;Kurobe M;Gotoh D;Ogawa T;Minagawa T;Ishizuka O;Chermansky C;Kaufman J;Yoshimura N;Tyagi P
在先前接受避水应激（WAS）和/或硫酸鱼精蛋白（PS）急性暴露的大鼠中，定量灌注造影剂混合物前后的膀胱壁T1弛豫时间（T1）。雌性Wistar大鼠随机接受假手术（对照）或连续10天1小时的WAS，然后在代谢笼中评价夜间排尿模式。在滴注4 mM钆布醇与5 mM Ferumoxytol的混合物之前和之后，在9.4 T下进行膀胱壁的T1映射。随后，在短暂的膀胱内PS暴露后重复T1映射或处死动物进行组织学和分析粘膜mRNA水平。与对照组相比，WAS暴露降低了单次排尿量，缩短了粘膜的造影后T1弛豫时间-用于计算相对较高的滴注钆布醇进入量。WAS组的渗透性受损由尿路上皮剥脱、水肿和ZO-1下调证实。PS暴露使两组中钆布醇的基线进入量加倍。这些研究结果证实，心理应激损害膀胱粘膜的细胞旁渗透性，其非侵入性测定与MRI通过PS曝光验证。
To quantify the urinary bladder wall T1 relaxation time (T1) before and after the instillation contrast mixture in rats previously subjected to water avoidance stress (WAS) and/or acute exposure to protamine sulfate (PS). Female Wistar rats were randomized to receive either sham (control) or 1 h of WAS for ten consecutive days before the evaluation of nocturnal urination pattern in metabolic cages. T1 mapping of urinary bladder wall at 9.4 T was performed pre- and post- instillation of 4 mM Gadobutrol in a mixture with 5 mM Ferumoxytol. Subsequently, either T1 mapping was repeated after brief intravesical PS exposure or the animals were sacrificed for histology and analyzing the mucosal levels of mRNA. Compared to the control group, WAS exposure decreased the single void urine volume and shortened the post-contrast T1 relaxation time of mucosa- used to compute relatively higher ingress of instilled Gadobutrol. Compromised permeability in WAS group was corroborated by the urothelial denudation, edema and ZO-1 downregulation. PS exposure doubled the baseline ingress of Gadobutrol in both groups. These findings confirm that psychological stress compromises the paracellular permeability of bladder mucosa and its non-invasive assay with MRI was validated by PS exposure.
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DOI：
10.1152/ajprenal.00307.2001
发表时间：
2002-08-01
影响因子：
4.2
作者：
Lavelle, J;Meyers, S;Zeidel, ML
通讯作者：
Zeidel, ML
影响因子：
2.5
作者：
Dewasmes, G;Loos, N;Ramadan, W
通讯作者：
Ramadan, W
影响因子：
6.6
作者：
Kim, Sung Han;Kim, Tae Beom;Oh, Seung-June
通讯作者：
Oh, Seung-June
影响因子：
2.1
作者：
Liu, Hsin-Tzu;Shie, Jia-Heng;Kuo, Hann-Chorng
通讯作者：
Kuo, Hann-Chorng
DOI：
10.1111/j.1464-410x.1983.tb03354.x
发表时间：
1983-01-01
期刊：
BRITISH JOURNAL OF UROLOGY
影响因子：
--
作者：
ELDRUP, J;THORUP, J;HAINAU, B
通讯作者：
HAINAU, B