Tracking neuronal motility in live murine retinal explants.
Tracking neuronal motility in live murine retinal explants.
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DOI：
10.1016/j.xpro.2021.101008
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Pearson RA
中科院分区：
文献类型：
--
作者：
Aghaizu ND;Warre-Cornish KM;Robinson MR;Ali RR;Pearson RA
发育中的视网膜经历动态的组织变化，包括周围细胞的显著视网膜内运动。在这里，我们提出了一个协议，用于跟踪视网膜细胞的运动在活的视网膜。虽然最初应用于视杆和视锥光感受器，但该策略适用于小鼠视网膜和其他类似实验视网膜模型中的任何荧光标记细胞。仔细的组织处理对于成功采集高质量的实时成像数据至关重要。提供了半自动计算机数据处理的进一步说明。有关本方案使用和执行的完整详细信息，请参阅。在活鼠视网膜外植体中追踪细胞运动性的方案使用转基因动物或通过转染对视网膜细胞进行荧光标记在双光子成像系统中对鼠视网膜进行实时成像设置视网膜细胞运动性的计算机追踪和定量发育中的视网膜经历动态组织变化，包括周围细胞的显著视网膜内运动性。在这里，我们提出了一个协议，用于跟踪视网膜细胞的运动在活的视网膜。虽然最初应用于视杆和视锥光感受器，但该策略适用于小鼠视网膜和其他类似实验视网膜模型中的任何荧光标记细胞。仔细的组织处理对于成功采集高质量的实时成像数据至关重要。提供了半自动计算机数据处理的进一步说明。
The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided. For complete details on the use and execution of this protocol, please refer to. Protocol for cell motility tracking in live murine retinal explants Fluorescent labelling of retinal cells using transgenic animals or by transfection Live-imaging set-up of murine retinae in a 2-photon imaging system In silico tracking and quantification of retinal cell motility The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided.
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DOI：
10.1073/pnas.0508214103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Akimoto, M;Cheng, H;Swaroop, A
通讯作者：
Swaroop, A
DOI：
10.1002/dvg.22918
发表时间：
2016-03
期刊：
Genesis (New York, N.Y. : 2000)
影响因子：
--
作者：
Brightman DS;Razafsky D;Potter C;Hodzic D;Chen S
通讯作者：
Chen S
影响因子：
64.8
作者：
Gong, SC;Zheng, C;Heintz, N
通讯作者：
Heintz, N
影响因子：
8.8
作者：
Aghaizu ND;Warre-Cornish KM;Robinson MR;Waldron PV;Maswood RN;Smith AJ;Ali RR;Pearson RA
通讯作者：
Pearson RA
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert