Repeated nuclear translocations underlie photoreceptor positioning and lamination of the outer nuclear layer in the mammalian retina.
Repeated nuclear translocations underlie photoreceptor positioning and lamination of the outer nuclear layer in the mammalian retina.
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DOI：
10.1016/j.celrep.2021.109461
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发表时间：
2021-08-03
期刊：
影响因子：
8.8
通讯作者：
Pearson RA
中科院分区：
文献类型：
--
作者：
Aghaizu ND;Warre-Cornish KM;Robinson MR;Waldron PV;Maswood RN;Smith AJ;Ali RR;Pearson RA
在发育过程中，几乎所有的分层神经元都必须从它们的出生地迁移到适当的神经层。光感受器位于视网膜的最顶端层，靠近它们的出生地。光感受器是否需要迁移事件用于精细定位和/或保留在该层内还没有很好地理解。在这里，我们表明，感光细胞核的发展中小鼠视网膜周期性地表现出快速的，动力蛋白-1依赖性易位向顶面，在基底方向移动更慢，可能是由于被动位移相邻的视网膜核。减弱动力蛋白1在视杆细胞中的功能导致其异位基底移位到外丛状层和内核层。在这些移位的细胞中，突触的形成也受到损害。我们建议，重复的，顶端定向核转位事件是必要的，以确保保留有丝分裂后的光感受器内新兴的外核层视网膜发生过程中，这是正确的神经元分层的关键。光感受器核在发育中的小鼠视网膜中表现出顶端-基底运动性快速顶端定向核移位由动力蛋白1驱动动力蛋白1破坏阻碍快速顶端核移位，导致移位在基底移位的光感受器中突触形成被破坏光感受器占据视网膜中的限定层。Aghaizu等人显示了哺乳动物视杆和视锥光感受器使用重复、动力蛋白-1驱动的顶端定向核易位来精细定位和保持在该层内。光感受器中的动力蛋白1破坏导致异位移位超出光感受器层和受损的突触形成。
In development, almost all stratified neurons must migrate from their birthplace to the appropriate neural layer. Photoreceptors reside in the most apical layer of the retina, near their place of birth. Whether photoreceptors require migratory events for fine-positioning and/or retention within this layer is not well understood. Here, we show that photoreceptor nuclei of the developing mouse retina cyclically exhibit rapid, dynein-1-dependent translocation toward the apical surface, before moving more slowly in the basal direction, likely due to passive displacement by neighboring retinal nuclei. Attenuating dynein 1 function in rod photoreceptors results in their ectopic basal displacement into the outer plexiform layer and inner nuclear layer. Synapse formation is also compromised in these displaced cells. We propose that repeated, apically directed nuclear translocation events are necessary to ensure retention of post-mitotic photoreceptors within the emerging outer nuclear layer during retinogenesis, which is critical for correct neuronal lamination. Photoreceptor nuclei exhibit apico-basal motility in the developing mouse retina Rapid apically directed nuclear translocation is powered by dynein 1 Dynein 1 disruption impedes rapid apical nuclear translocation, causing displacement Synapse formation is disrupted in basally displaced photoreceptors Photoreceptors occupy a defined layer in the retina. Aghaizu et al. show that mammalian rod and cone photoreceptors use repeated, dynein-1-driven apically directed nuclear translocations for fine-positioning and retention within this layer. Dynein 1 disruption in photoreceptors results in ectopic displacement beyond the photoreceptor layer and impaired synapse formation.
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DOI：
10.1083/jcb.201004118
发表时间：
2010-10-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fridolfsson HN;Starr DA
通讯作者：
Starr DA
影响因子：
56.9
作者：
Famulski, Jakub K.;Trivedi, Niraj;Solecki, David J.
通讯作者：
Solecki, David J.
影响因子：
4.6
作者：
Edqvist, PHD;Hallböök, F
通讯作者：
Hallböök, F
影响因子：
7.8
作者：
Icha, Jaroslav;Kunath, Christiane;Norden, Caren
通讯作者：
Norden, Caren
DOI：
10.1073/pnas.0508214103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Akimoto, M;Cheng, H;Swaroop, A
通讯作者：
Swaroop, A