Plasmodium falciparum: differential selection of drug resistance alleles in contiguous urban and peri-urban areas of Brazzaville, Republic of Congo.
Plasmodium falciparum: differential selection of drug resistance alleles in contiguous urban and peri-urban areas of Brazzaville, Republic of Congo.
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DOI：
10.1371/journal.pone.0023430
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Culleton R
中科院分区：
文献类型：
--
作者：
Tsumori Y;Ndounga M;Sunahara T;Hayashida N;Inoue M;Nakazawa S;Casimiro P;Isozumi R;Uemura H;Tanabe K;Kaneko O;Culleton R
非洲大陆目前正经历着人口的快速增长，城市化程度的提高使居住在大城镇的人口比例增加。我们研究了城市化程度对刚果共和国布拉柴维尔城市及其周边地区恶性疟原虫群体遗传学的影响。这个领域的设置，其中包括当地的卫生中心位于不同的城市化地区，是感兴趣的，因为它允许从人类，寄生虫和蚊子种群共享的地区的疟疾寄生虫的特性，但在城市化程度的差异（导致传播强度的巨大差异）导致疟疾传播的模式有很大的不同。我们调查了这些传播强度的差异如何影响寄生虫的遗传多样性，包括每个地区的遗传多态性的数量，人群内的连锁不平衡程度，以及耐药标记的流行和频率。为了确定寄生虫种群结构，杂合性和连锁不平衡，我们分型8个微卫星标记，并进行单倍型分析的msp1基因的PCR。已知与抗疟药氯喹和乙胺嘧啶耐药性相关的突变分别通过对crt和dhfr基因的相关部分进行测序来确定。我们发现，寄生虫的遗传多样性是可比的两个网站之间，保持在两个领域的高水平的多态性，尽管在传输强度显着差异。至关重要的是，我们发现，对乙胺嘧啶和氯喹的耐药性的遗传标记的频率显着不同的网站之间，在这两个地区不同的选择压力的指示。
The African continent is currently experiencing rapid population growth, with rising urbanization increasing the percentage of the population living in large towns and cities. We studied the impact of the degree of urbanization on the population genetics of Plasmodium falciparum in urban and peri-urban areas in and around the city of Brazzaville, Republic of Congo. This field setting, which incorporates local health centers situated in areas of varying urbanization, is of interest as it allows the characterization of malaria parasites from areas where the human, parasite, and mosquito populations are shared, but where differences in the degree of urbanization (leading to dramatic differences in transmission intensity) cause the pattern of malaria transmission to differ greatly. We have investigated how these differences in transmission intensity affect parasite genetic diversity, including the amount of genetic polymorphism in each area, the degree of linkage disequilibrium within the populations, and the prevalence and frequency of drug resistance markers. To determine parasite population structure, heterozygosity and linkage disequilibrium, we typed eight microsatellite markers and performed haplotype analysis of the msp1 gene by PCR. Mutations known to be associated with resistance to the antimalarial drugs chloroquine and pyrimethamine were determined by sequencing the relevant portions of the crt and dhfr genes, respectively. We found that parasite genetic diversity was comparable between the two sites, with high levels of polymorphism being maintained in both areas despite dramatic differences in transmission intensity. Crucially, we found that the frequencies of genetic markers of drug resistance against pyrimethamine and chloroquine differed significantly between the sites, indicative of differing selection pressures in the two areas.
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临床免疫宿主是对药物敏感疟疾寄生虫的避难所。
影响因子：
3
作者：
Klein EY;Smith DL;Boni MF;Laxminarayan R
通讯作者：
Laxminarayan R
影响因子：
6.7
作者：
Barry AE;Leliwa-Sytek A;Tavul L;Imrie H;Migot-Nabias F;Brown SM;McVean GA;Day KP
通讯作者：
Day KP
DOI：
10.4269/ajtmh.1989.41.259
发表时间：
1989-09-01
影响因子：
3.3
作者：
FORSYTH, KP;PHILIP, G;BROWN, GV
通讯作者：
BROWN, GV
DOI：
10.4269/ajtmh.1996.54.632
发表时间：
1996-06-01
影响因子：
3.3
作者：
Contamin, H;Fandeur, T;MercereauPuijalon, O
通讯作者：
MercereauPuijalon, O
影响因子：
3
作者：
Culleton, Richard L.;Mita, Toshihiro;Tanabe, Kazuyuki
通讯作者：
Tanabe, Kazuyuki