Genome-Wide Characterization and Expression Profiling of the GRAS Gene Family in Salt and Alkali Stresses in Miscanthus sinensis.
Genome-Wide Characterization and Expression Profiling of the GRAS Gene Family in Salt and Alkali Stresses in Miscanthus sinensis.
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DOI：
10.3390/ijms232314521
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发表时间：
2022-11-22
影响因子：
5.6
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中科院分区：
文献类型：
--
作者：
GRAS 家族基因编码植物特异性转录因子，在多种发育过程和非生物胁迫反应中发挥重要作用。然而，生物能源作物芒草中GRAS基因家族的信息尚未获得。在这里，我们报告了芒草中 GRAS 基因家族的全基因组鉴定。共鉴定出123个MsGRAS基因，根据系统发育分析将其分为10个亚家族。共线性分析显示，59 个 MsGRAS 基因经历了片段复制，形成 35 个旁系同源对。通过转录组和实时定量 PCR (RT-qPCR) 分析，分析了 6 个 MsGRAS 基因在盐、碱和混合盐碱胁迫下的表达情况。此外，在转基因拟南芥中对 MsGRAS60 在盐和碱胁迫反应中的作用进行了表征。 MsGRAS60 过表达株系对脱落酸 (ABA) 处理表现出不敏感性，并导致对盐和碱胁迫的耐受性受损，表明 MsGRAS60 通过 ABA 依赖性信号通路是盐和碱耐受性的负调节因子。鉴定出的盐和碱胁迫诱导型 MsGRAS 基因可作为改善芒草非生物胁迫耐受性的候选基因。
The GRAS family genes encode plant-specific transcription factors that play important roles in a diverse range of developmental processes and abiotic stress responses. However, the information of GRAS gene family in the bioenergy crop Miscanthus has not been available. Here, we report the genome-wide identification of GRAS gene family in Micanthus sinensis. A total of 123 MsGRAS genes were identified, which were divided into ten subfamilies based on the phylogenetic analysis. The co-linearity analysis revealed that 59 MsGRAS genes experienced segmental duplication, forming 35 paralogous pairs. The expression of six MsGRAS genes in responding to salt, alkali, and mixed salt-alkali stresses was analyzed by transcriptome and real-time quantitative PCR (RT-qPCR) assays. Furthermore, the role of MsGRAS60 in salt and alkali stress response was characterized in transgenic Arabidopsis. The MsGRAS60 overexpression lines exhibited hyposensitivity to abscisic acid (ABA) treatment and resulted in compromised tolerance to salt and alkali stresses, suggesting that MsGRAS60 is a negative regulator of salt and alkali tolerance via an ABA-dependent signaling pathway. The salt and alkali stress-inducible MsGRAS genes identified serve as candidates for the improvement of abiotic stress tolerance in Miscanthus.
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