The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in Arabidopsis thaliana.

The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in Arabidopsis thaliana.
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盐和干旱诱导的杨树 GRAS 蛋白 SCL7 赋予拟南芥耐盐和干旱的能力

DOI:
10.1093/jxb/erq217
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发表时间:
2010-09
影响因子:
6.9
通讯作者:
Yin WL
Yin WL
中科院分区:
生物学1区
文献类型:
--
作者:
Ma HS;Liang D;Shuai P;Xia XL;Yin WL

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植物特异性GRAS/SCL转录因子在植物发育和逆境应答中发挥着不同的作用。在这项研究中,白杨SCL基因,PeSCL 7,在拟南芥的功能特点,特别是关于其在非生物胁迫抗性的作用。在白杨中的表达分析表明,PeSCL 7诱导干旱和高盐胁迫,但抑制赤霉素(GA)处理的叶片。GFP-PeSCL 7在洋葱表皮细胞中的瞬时表达揭示了PeSCL 7蛋白定位于细胞核中。过表达PeSCL 7的转基因拟南芥表现出对干旱和盐处理的耐受性增强。两种胁迫响应酶的活性在转基因幼苗中增加。两者合计,这些结果表明,PeSCL 7编码的压力响应的GRAS/SCL转录因子,这是潜在的有用的工程耐旱和耐盐的树木的成员。
The plant-specific GRAS/SCL transcription factors play diverse roles in plant development and stress responses. In this study, a poplar SCL gene, PeSCL7, was functionally characterized in Arabidopsis thaliana, especially with regard to its role in abiotic stress resistance. Expression analysis in poplar revealed that PeSCL7 was induced by drought and high salt stresses, but was repressed by gibberellic acid (GA) treatment in leaves. Transient expression of GFP-PeSCL7 in onion epidermal cells revealed that the PeSCL7 protein was localized in the nucleus. Transgenic Arabidopsis plants overexpressing PeSCL7 showed enhanced tolerance to drought and salt treatments. The activity of two stress-responsive enzymes was increased in transgenic seedlings. Taken together, these results suggest that PeSCL7 encodes a member of the stress-responsive GRAS/SCL transcription factors that is potentially useful for engineering drought- and salt-tolerant trees.
DOI: 10.1105/tpc.007096
发表时间: 2003-01-01
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