The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice.
The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice.
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DOI：
10.3390/life11010007
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发表时间：
2020-12-24
期刊：
影响因子：
--
通讯作者：
Ma TY
中科院分区：
文献类型：
--
作者：
LeBrun ES;Nighot M;Dharmaprakash V;Kumar A;Lo CC;Chain PSG;Ma TY
在人类以及包括小鼠在内的其他几种哺乳动物中，已经对饮酒对健康的影响（包括积极和消极的影响）进行了充分的研究。许多研究也将这些相同的健康影响和表型与肠道微生物群落的特定成员联系起来。在这里，我们利用慢性加暴饮乙醇饲料模型（高暴饮模型）来探索在小鼠中进行的三个独立实验中微生物群落的变化。我们发现，在不含乙醇的相同饮食中，经乙醇处理的小鼠和对照组小鼠之间，微生物群落的组成存在显著且可重复的差异。我们还发现了乙醇处理后肠道菌群的暂时性显著差异。在开始每日饮酒后4天观察到社区的高峰转变。我们定量鉴定了许多指示这些乙醇诱导转移的细菌属，其中包括与对照比较乙醇处理时的20个重要属和基于时间调查的14个重要属。包括处理与时间变化的重叠，我们确定了乙醇处理微生物组变化的25个特定属。移位与观察到的肝组织中脂肪沉积的表现一致，即酒精性肝病相关表型。本文提出的证据来自三个独立的实验，表明存在一个共同的、可重复的、可表征的“小鼠乙醇肠道微生物群”。
Phenotypic health effects, both positive and negative, have been well studied in association with the consumption of alcohol in humans as well as several other mammals including mice. Many studies have also associated these same health effects and phenotypes to specific members of gut microbiome communities. Here we utilized a chronic plus binge ethanol feed model (Gao-binge model) to explore microbiome community changes across three independent experiments performed in mice. We found significant and reproducible differences in microbiome community assemblies between ethanol-treated mice and control mice on the same diet absent of ethanol. We also identified significant differences in gut microbiota occurring temporally with ethanol treatment. Peak shift in communities was observed 4 days after the start of daily alcohol consumption. We quantitatively identified many of the bacterial genera indicative of these ethanol-induced shifts including 20 significant genera when comparing ethanol treatments with controls and 14 significant genera based on temporal investigation. Including overlap of treatment with temporal shifts, we identified 25 specific genera of interest in ethanol treatment microbiome shifts. Shifts coincide with observed presentation of fatty deposits in the liver tissue, i.e., Alcoholic Liver Disease-associated phenotype. The evidence presented herein, derived from three independent experiments, points to the existence of a common, reproducible, and characterizable “mouse ethanol gut microbiome”.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.3
作者：
Hold, Georgina L.;Smith, Megan;Mukhopadhya, Indrani
通讯作者：
Mukhopadhya, Indrani
DOI：
10.1111/j.1530-0277.2011.01487.x
发表时间：
2011-08
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Inokuchi S;Tsukamoto H;Park E;Liu ZX;Brenner DA;Seki E
通讯作者：
Seki E
影响因子：
24.5
作者：
Grander, Christoph;Adolph, Timon E.;Tilg, Herbert
通讯作者：
Tilg, Herbert
DOI：
10.1152/ajpgi.90232.2008
发表时间：
2008-10-01
影响因子：
4.5
作者：
Gobejishvili, Leila;Barve, Shirish;McClain, Craig
通讯作者：
McClain, Craig