Cross-fostering immediately after birth induces a permanent microbiota shift that is shaped by the nursing mother.
Cross-fostering immediately after birth induces a permanent microbiota shift that is shaped by the nursing mother.
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出生后立即进行交叉促进会引起由哺乳母亲塑造的永久性菌群转移。
DOI：
10.1186/s40168-015-0080-y
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发表时间：
2015
期刊：
影响因子：
15.5
通讯作者：
Lorenz RG
中科院分区：
文献类型：
--
作者：
Daft JG;Ptacek T;Kumar R;Morrow C;Lorenz RG
目前的研究已经使人们认识到，健康个体和易患病个体之间的共生微生物群存在差异。目前正在探索通过操纵胃肠道微生物群来逆转疾病发病机制的治疗方法。在同一研究中，不同小鼠品系之间的微生物群的正常化也需要更好地了解疾病的发病机制。目前的方法需要反复输送细菌和大量动物，并且治疗开始时间各不相同。需要一种方法，可以在早期将易感个体的微生物群转变为健康的微生物群，并在个体的一生中维持这种转变。我们测试了在出生后48小时内进行交叉饲养的幼崽，作为一种永久改变出生时微生物群的手段。分类学分析显示，哺乳母亲是决定细菌定植的关键因素，而不是生母。使用粪便颗粒中的细菌16S rDNA序列对数据进行评估，并在Illumina Miseq上使用251 bp的对端文库进行测序。结果表明，交叉培养是诱导共生菌群发生早期、持续转移的有效手段。这将允许评估长期的微生物转移及其对疾病发病机制的影响。交叉培养还将通过使不同品系小鼠之间的共生菌群正常化，消除控制模型内的变异。本文的在线版本（doi:10.1186/s40168-015-0080-y）包含补充材料，仅供授权用户使用。
Current research has led to the appreciation that there are differences in the commensal microbiota between healthy individuals and individuals that are predisposed to disease. Treatments to reverse disease pathogenesis through the manipulation of the gastrointestinal (GI) microbiota are now being explored. Normalizing microbiota between different strains of mice in the same study is also needed to better understand disease pathogenesis. Current approaches require repeated delivery of bacteria and large numbers of animals and vary in treatment start time. A method is needed that can shift the microbiota of predisposed individuals to a healthy microbiota at an early age and sustain this shift through the lifetime of the individual. We tested cross-fostering of pups within 48 h of birth as a means to permanently shift the microbiota from birth. Taxonomical analysis revealed that the nursing mother was the critical factor in determining bacterial colonization, instead of the birth mother. Data was evaluated using bacterial 16S rDNA sequences from fecal pellets and sequencing was performed on an Illumina Miseq using a 251 bp paired-end library. The results show that cross-fostering is an effective means to induce an early and maintained shift in the commensal microbiota. This will allow for the evaluation of a prolonged microbial shift and its effects on disease pathogenesis. Cross-fostering will also eliminate variation within control models by normalizing the commensal microbiota between different strains of mice. The online version of this article (doi:10.1186/s40168-015-0080-y) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Rogers GB;Kozlowska J;Keeble J;Metcalfe K;Fao M;Dowd SE;Mason AJ;McGuckin MA;Bruce KD
通讯作者：
Bruce KD
影响因子：
3.7
作者：
Chen W;Liu F;Ling Z;Tong X;Xiang C
通讯作者：
Xiang C
DOI：
10.1128/genomea.00705-14
发表时间：
2014-07-17
期刊：
Genome announcements
影响因子：
--
作者：
Bolotin A;de Wouters T;Schnupf P;Bouchier C;Loux V;Rhimi M;Jamet A;Dervyn R;Boudebbouze S;Blottière HM;Sorokin A;Snel J;Cerf-Bensussan N;Gaboriau-Routhiau V;van de Guchte M;Maguin E
通讯作者：
Maguin E
影响因子：
56.9
作者：
Markle, Janet G. M.;Frank, Daniel N.;Danska, Jayne S.
通讯作者：
Danska, Jayne S.
影响因子：
4.4
作者：
Hansen, Camilla H. F.;Andersen, Line S. F.;Hansen, Axel K.
通讯作者：
Hansen, Axel K.