Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
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DOI：
10.1371/journal.pone.0039743
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xiang C
中科院分区：
文献类型：
--
作者：
Chen W;Liu F;Ling Z;Tong X;Xiang C
最近的报告表明肠道微生物群参与结直肠癌（CRC）的进展。我们利用基于焦磷酸测序的16 S rRNA基因分析来确定结直肠癌患者和健康对照者微生物群的整体结构;我们研究了肠腔、癌组织和匹配的非癌正常组织的微生物群。此外，我们使用直肠拭子样本研究了粘膜粘附微生物组成，因为组织粘附细菌群落的结构在肠道清洁后可能会发生变化。我们的研究结果表明，肠腔和癌组织的微生物结构显着不同。增强从饮食中获取能量或与宿主进行代谢交换的系统型在管腔中更丰富。管腔内厚壁菌门数量较多，拟杆菌门和变形菌门数量较少。癌组织和非癌组织的总体微生物结构相似;然而，肿瘤微生物群表现出较低的多样性。与健康个体中匹配的微生物群相比，CRC患者的肠腔微生物群和粘膜粘附微生物群的结构有所不同。乳酸杆菌在癌组织中富集，而粪杆菌减少。在结直肠癌患者的粘膜粘附微生物群中，双歧杆菌、粪杆菌和布劳特氏菌减少，而梭杆菌、卟啉单胞菌、消化链球菌和莫吉杆菌富集。在管腔中，与代谢紊乱或与宿主的代谢交换有关的主要代谢类型，丹毒菌科，普雷沃菌科和Coriobacteriaceae在癌症患者中增加。结合先前的报告，这些结果表明肠道微生物群与CRC风险相关，并且肠腔微生物群可能通过彗星或与宿主的代谢交换影响CRC风险。然而，粘膜相关微生物群主要通过与宿主的直接相互作用潜在地影响CRC风险。
Recent reports have suggested the involvement of gut microbiota in the progression of colorectal cancer (CRC). We utilized pyrosequencing based analysis of 16S rRNA genes to determine the overall structure of microbiota in patients with colorectal cancer and healthy controls; we investigated microbiota of the intestinal lumen, the cancerous tissue and matched noncancerous normal tissue. Moreover, we investigated the mucosa-adherent microbial composition using rectal swab samples because the structure of the tissue-adherent bacterial community is potentially altered following bowel cleansing. Our findings indicated that the microbial structure of the intestinal lumen and cancerous tissue differed significantly. Phylotypes that enhance energy harvest from diets or perform metabolic exchange with the host were more abundant in the lumen. There were more abundant Firmicutes and less abundant Bacteroidetes and Proteobacteria in lumen. The overall microbial structures of cancerous tissue and noncancerous tissue were similar; howerer the tumor microbiota exhibited lower diversity. The structures of the intestinal lumen microbiota and mucosa-adherent microbiota were different in CRC patients compared to matched microbiota in healthy individuals. Lactobacillales was enriched in cancerous tissue, whereas Faecalibacterium was reduced. In the mucosa-adherent microbiota, Bifidobacterium, Faecalibacterium, and Blautia were reduced in CRC patients, whereas Fusobacterium, Porphyromonas, Peptostreptococcus, and Mogibacterium were enriched. In the lumen, predominant phylotypes related to metabolic disorders or metabolic exchange with the host, Erysipelotrichaceae, Prevotellaceae, and Coriobacteriaceae were increased in cancer patients. Coupled with previous reports, these results suggest that the intestinal microbiota is associated with CRC risk and that intestinal lumen microflora potentially influence CRC risk via cometabolism or metabolic exchange with the host. However, mucosa-associated microbiota potentially affects CRC risk primarily through direct interaction with the host.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
--
作者：
Harris, JI;Russell, RRB;Taylor, JJ
通讯作者：
Taylor, JJ
影响因子：
3.7
作者：
Domenici E;Willé DR;Tozzi F;Prokopenko I;Miller S;McKeown A;Brittain C;Rujescu D;Giegling I;Turck CW;Holsboer F;Bullmore ET;Middleton L;Merlo-Pich E;Alexander RC;Muglia P
通讯作者：
Muglia P
定植引起的宿主微生物代谢相互作用。
影响因子：
6.4
作者：
Claus SP;Ellero SL;Berger B;Krause L;Bruttin A;Molina J;Paris A;Want EJ;de Waziers I;Cloarec O;Richards SE;Wang Y;Dumas ME;Ross A;Rezzi S;Kochhar S;Van Bladeren P;Lindon JC;Holmes E;Nicholson JK
通讯作者：
Nicholson JK
影响因子：
13.5
作者：
Chen, Yanfei;Yang, Fengling;Li, Lanjuan
通讯作者：
Li, Lanjuan