Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research.
Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research.
复制标题
DOI：
10.1016/j.scr.2021.102409
复制
发表时间：
2021-07
影响因子：
1.2
通讯作者：
Clark, Amander T.
中科院分区：
文献类型：
--
作者：
Pandolfi, Erica C.;Hunt, Timothy J.;Goldsmith, Sierra;Hurlbut, Kellie;Silber, Sherman J.;Clark, Amander T.
我们从人类真皮成纤维细胞(HDF)(MZT05)中产生了三个人类诱导多能干细胞(HiPSC)亚系，该细胞来自一名以前有生育能力的妇女捐赠的皮肤活检。皮肤活检被广泛同意用于生物医学研究，包括专门用于研究人类生育、不孕和生殖细胞分化的唯一同意。使用仙台病毒载体对HiPSCs进行重新编程，随后检测到自我更新的标志呈阳性。用PluriTest分析进一步验证多能性，并用Taqman Real-time PCR检测体外分化。这些亚系作为hiPSC研究项目的对照，旨在了解女性生育的细胞和分子调控。
We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Pluripotency was further verified using PluriTest analysis and in vitro differentiation was tested using Taqman Real-Time PCR assays. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility.
登录
查看更多内容
影响因子：
3.5
作者：
Perez, Silvia V. Diaz;Kim, Rachel;Clark, Amander T.
通讯作者：
Clark, Amander T.
影响因子：
1.2
作者：
Pandolfi, Erica C.;Rojas, Ernesto J.;Clark, Amander T.
通讯作者：
Clark, Amander T.
影响因子：
5
作者：
Tokusumi, T;Iida, A;Hasegawa, M
通讯作者：
Hasegawa, M
影响因子：
48
作者：
Mueller, Franz-Josef;Schuldt, Bernhard M.;Williams, Roy;Mason, Dylan;Altun, Gulsah;Papapetrou, Eirini P.;Danner, Sandra;Goldmann, Johanna E.;Herbst, Arne;Schmidt, Nils O.;Aldenhoff, Josef B.;Laurent, Louise C.;Loring, Jeanne F.
通讯作者：
Loring, Jeanne F.