Retraction of DNA-bound type IV competence pili initiates DNA uptake during natural transformation in Vibrio cholerae.
Retraction of DNA-bound type IV competence pili initiates DNA uptake during natural transformation in Vibrio cholerae.
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DOI：
10.1038/s41564-018-0174-y
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发表时间：
2018-07
影响因子：
28.3
通讯作者：
Dalia AB
中科院分区：
文献类型：
--
作者：
Ellison CK;Dalia TN;Vidal Ceballos A;Wang JC;Biais N;Brun YV;Dalia AB
自然转化是细菌物种中广泛保守的水平基因转移机制，可以塑造进化并促进抗生素抗性决定簇的传播，促进抗原变异并导致获得新的毒力因子。被称为感受态皮利的表面附属物在自然转化的第一步促进DNA摄取;然而，由于缺乏在活细胞中可视化这些结构的方法，它们的作用机制仍然不清楚。在这里，使用模型自然transformable speciesVibrio cholerae和菌毛标记的方法，我们定义的机制IV型感受态菌毛介导的DNA摄取过程中的自然转化。首先，我们表明，IV型竞争力皮利通过其尖端结合到细胞外双链DNA，并证明这种结合是DNA摄取的关键。接下来，我们表明，IV型能力皮利是动态结构，菌毛收缩带来的尖端结合的DNA细胞表面。最后，我们表明，菌毛回缩是时空耦合到DNA内化和空间阻碍菌毛回缩防止DNA摄取。总之，这些结果表明，IV型感受态皮利通过其尖端直接与DNA结合，并在这种保守的水平基因转移机制中通过回缩介导DNA内化。
Natural transformation is a broadly conserved mechanism of horizontal gene transfer in bacterial species that can shape evolution and foster the spread of antibiotic resistance determinants, promote antigenic variation and lead to the acquisition of novel virulence factors. Surface appendages called competence pili promote DNA uptake during the first step of natural transformation; however, their mechanism of action has remained unclear owing to an absence of methods to visualize these structures in live cells. Here, using the model naturally transformable speciesVibrio choleraeand a pilus-labelling method, we define the mechanism for type IV competence pilus-mediated DNA uptake during natural transformation. First, we show that type IV competence pili bind to extracellular double-stranded DNA via their tip and demonstrate that this binding is critical for DNA uptake. Next, we show that type IV competence pili are dynamic structures and that pilus retraction brings tip-bound DNA to the cell surface. Finally, we show that pilus retraction is spatiotemporally coupled to DNA internalization and that sterically obstructing pilus retraction prevents DNA uptake. Together, these results indicate that type IV competence pili directly bind to DNA via their tip and mediate DNA internalization through retraction during this conserved mechanism of horizontal gene transfer.
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DOI：
10.1126/science.aad2001
发表时间：
2016-03-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chang YW;Rettberg LA;Treuner-Lange A;Iwasa J;Søgaard-Andersen L;Jensen GJ
通讯作者：
Jensen GJ
影响因子：
4.5
作者：
Lo Scrudato M;Blokesch M
通讯作者：
Blokesch M
影响因子：
14.9
作者：
Lo Scrudato M;Blokesch M
通讯作者：
Blokesch M
影响因子：
6.7
作者：
Gangel H;Hepp C;Müller S;Oldewurtel ER;Aas FE;Koomey M;Maier B
通讯作者：
Maier B
DOI：
10.1073/pnas.1218832110
发表时间：
2013-02-19
影响因子：
11.1
作者：
Cehovin, Ana;Simpson, Peter J.;Pelicic, Vladimir
通讯作者：
Pelicic, Vladimir