e-Cigarette Vapour Condensate Reduces Viability and Impairs Function of Human Osteoblasts, in Part, via a Nicotine Dependent Mechanism.
e-Cigarette Vapour Condensate Reduces Viability and Impairs Function of Human Osteoblasts, in Part, via a Nicotine Dependent Mechanism.
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DOI：
10.3390/toxics10090506
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发表时间：
2022-08-28
期刊：
影响因子：
4.6
通讯作者：
Jones SW
中科院分区：
文献类型：
--
作者：
Nicholson T;Davis L;Davis ET;Newton Ede M;Scott A;Jones SW
吸烟对骨骼质量有负面影响，是多种骨骼相关疾病发展的风险因素，因为骨骼的高度血管化结构暴露在全身因素中。然而，人们对电子烟(电子烟)对骨骼的影响知之甚少，因为电子烟含有高剂量的尼古丁和其他化合物，包括调味剂、金属颗粒和羰基。在这里，我们提出了第一个证据，证明了电子烟蒸气冷凝物(复制电子烟液体化学结构在设备使用时发生的变化)对人类原代成骨细胞活性和功能的影响。成骨细胞暴露于第二代或第三代设备产生的电子烟蒸气冷凝物24小时，成骨细胞活力显著降低，并呈剂量依赖关系，其中更强大的第三代设备产生的冷凝物具有更大的毒性。这种影响部分是由尼古丁介导的，因为暴露在相同浓度的无尼古丁冷凝物中毒性较小。电子烟蒸气冷凝物对成骨细胞活性的有害影响通过与抗氧化剂N-乙酰-L-半胱氨酸(NAC)共同处理而得到缓解，这表明毒性也可能是由设备使用时产生的活性物质驱动的。最后，无毒剂量的第二代或第三代冷凝物在24小时后显著钝化成骨细胞护骨素的分泌，这种作用可持续长达7天。综上所述，我们证明了由常用的第二代和第三代设备产生的电子烟蒸气冷凝物在生理上相关的剂量下可以显著降低成骨细胞的活性并损害成骨细胞的功能。这些数据突显了进一步调查的必要性，以告知用户使用电子烟对骨骼健康的潜在风险，包括加速骨骼相关疾病的进展，影响年轻用户的骨骼发育，并建议骨科手术、牙科手术或受伤后的患者最大限度地促进骨骼愈合。
Cigarette consumption negatively impacts bone quality and is a risk-factor for the development of multiple bone associated disorders, due to the highly vascularised structure of bone being exposed to systemic factors. However, the impact on bone to electronic cigarette (e-cigarette) use, which contains high doses of nicotine and other compounds including flavouring chemicals, metal particulates and carbonyls, is poorly understood. Here, we present the first evidence demonstrating the impact of e-cigarette vapour condensate (replicating changes in e-cigarette liquid chemical structure that occur upon device usage), on human primary osteoblast viability and function. 24 h exposure of osteoblasts to e-cigarette vapour condensate, generated from either second or third generation devices, significantly reduced osteoblast viability in a dose dependent manner, with condensate generated from the more powerful third generation device having greater toxicity. This effect was mediated in-part by nicotine, since exposure to nicotine-free condensate of an equal concentration had a less toxic effect. The detrimental effect of e-cigarette vapour condensate on osteoblast viability was rescued by co-treatment with the antioxidant N-Acetyl-L-cysteine (NAC), indicating toxicity may also be driven by reactive species generated upon device usage. Finally, non-toxic doses of either second or third generation condensate significantly blunted osteoblast osteoprotegerin secretion after 24 h, which was sustained for up to 7 days. In summary we demonstrate that e-cigarette vapour condensate, generated from commonly used second and third generation devices, can significantly reduce osteoblast viability and impair osteoblast function, at physiologically relevant doses. These data highlight the need for further investigation to inform users of the potential risks of e-cigarette use on bone health, including, accelerating bone associated disease progression, impacting skeletal development in younger users and to advise patients following orthopaedic surgery, dental surgery, or injury to maximise bone healing.
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DOI：
10.1164/rccm.201903-0615oc
发表时间：
2019-12-01
影响因子：
24.7
作者：
Ghosh, Arunava;Coakley, Raymond D.;Tarran, Robert
通讯作者：
Tarran, Robert
影响因子：
105.7
作者：
Law, MR;Hackshaw, AK
通讯作者：
Hackshaw, AK
影响因子：
6.7
作者：
Lappin, David F.;Sherrabeh, Sakhr;Macpherson, Lorna M. D.
通讯作者：
Macpherson, Lorna M. D.
影响因子：
4.7
作者：
Cheng, Guang;Guo, Jiehong;Hecht, Stephen S.
通讯作者：
Hecht, Stephen S.
影响因子：
2.3
作者：
Hiler, Marzena;Karaoghlanian, Nareg;Talih, Soha;Maloney, Sarah;Breland, Alison;Shihadeh, Alan;Eissenberg, Thomas
通讯作者：
Eissenberg, Thomas