Tim-4 expressing monocytes as a novel indicator to assess disease activity and severity of ulcerative colitis.
Tim-4 expressing monocytes as a novel indicator to assess disease activity and severity of ulcerative colitis.
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表达 Tim-4 的单核细胞作为评估溃疡性结肠炎疾病活动性和严重程度的新指标。
DOI：
10.1016/j.lfs.2021.119077
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发表时间：
2021-01
期刊：
影响因子：
6.1
通讯作者：
Yong Xie
中科院分区：
文献类型：
--
作者：
Junming Li;Guohui Xue;Meijun Zhong;Bin Zhou;Lin Hua;Yuanwang Chen;Dongsheng Liu;Yong Xie
目的免疫反应失调已被证明与溃疡性结肠炎（UC）的发病机制有关。 Tim-4 是免疫系统的潜在调节剂，在多种自身免疫性疾病中发挥着关键作用。然而，其是否与UC有关尚不清楚。本研究的目的是确定Tim-4在UC患者循环单核细胞上的表达及其临床意义。主要方法本研究共纳入36名UC患者和34名健康对照（HC）。通过流式细胞术测定外周血中CD14+Tim-4+细胞、调节性T细胞(Treg)和CD14+HLA-DR-/低髓源性抑制细胞(MDSC)的频率。通过化学发光免疫分析法测定血清 IL-6 水平。 主要发现 UC 患者中 CD14+Tim-4+ 细胞的百分比高于 HC 患者。 UC患者中Treg细胞的频率显着降低，而MDSC的频率显着增加。在严重程度高、每天排便次数多、UC疾病活动指数Mayo评分高、IgG高和炎症标志物水平高的受试者中，CD14+Tim-4+细胞的频率显着升高。接受美沙拉嗪治疗 3 周的 UC 患者中表达 Tim-4 的单核细胞百分比显着下降。此外，CD14+Tim-4+细胞的频率还与MDSC呈正相关，与Treg细胞呈负相关。意义CD14+Tim-4+细胞在UC患者中升高，可能成为评估UC疾病严重程度和活动性的新指标。
AimsThe dysregulation of the immune response has been shown to be involved in ulcerative colitis (UC) pathogenesis. Tim-4 is a potential regulator of the immune system which plays key roles in multiple autoimmune diseases. However, whether it is involved in UC remains unclear. The aim of this research was to determine the expression of Tim-4 on circulating monocytes and its clinical significance in UC patients.Main methodsIn total, 36 UC patients and 34 healthy controls (HCs) were enrolled in this study. The frequencies of CD14+Tim-4+cells, regulatory T cells (Treg) and CD14+HLA-DR−/lowmyeloid-derived suppressor cells (MDSCs) in the peripheral blood were determined by flow cytometry. Serum IL-6 levels were determined by chemiluminescence immunoassay.Key findingsThe percentage of CD14+Tim-4+cells was higher in UC patients than in HCs. The frequency of Treg cells was significantly decreased, while that of MDSCs was significantly increased in UC patients. The frequency of CD14+Tim-4+cells was significantly elevated in subjects with high severity, high number of defecations per day, high UC disease activity index Mayo score, high IgG, and high levels of inflammatory markers. And the percentages of Tim-4-expressing monocytes were significantly decreased in UC patients that received a 3-week treatment with mesalazine. Furthermore, the frequency of CD14+Tim-4+cells was also positively correlated with MDSCs and negatively correlated with Treg cells.SignificanceCD14+Tim-4+cells was elevated in UC patients and could be a novel indicator to assess disease severity and activity of UC.
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影响因子：
5.9
作者：
Wark G;Samocha-Bonet D;Ghaly S;Danta M
通讯作者：
Danta M
肝脏缺血预处理通过减少 TIM4 表达减轻缺血再灌注损伤
影响因子：
9.2
作者：
Zhang Y;Shen Q;Liu Y;Chen H;Zheng X;Xie S;Ji H;Zheng S
通讯作者：
Zheng S
影响因子：
30.3
作者：
Schirmer M;Denson L;Vlamakis H;Franzosa EA;Thomas S;Gotman NM;Rufo P;Baker SS;Sauer C;Markowitz J;Pfefferkorn M;Oliva-Hemker M;Rosh J;Otley A;Boyle B;Mack D;Baldassano R;Keljo D;LeLeiko N;Heyman M;Griffiths A;Patel AS;Noe J;Kugathasan S;Walters T;Huttenhower C;Hyams J;Xavier RJ
通讯作者：
Xavier RJ
DOI：
10.1073/pnas.0910359107
发表时间：
2010-05-11
影响因子：
11.1
作者：
Rodriguez-Manzanet, Roselynn;Sanjuan, Miguel A.;Kuchroo, Vijay K.
通讯作者：
Kuchroo, Vijay K.
影响因子：
16.6
作者：
Haberman, Yael;Karns, Rebekah;Denson, Lee A.
通讯作者：
Denson, Lee A.