Translocation and Dissemination of Gut Bacteria after Severe Traumatic Brain Injury.
Translocation and Dissemination of Gut Bacteria after Severe Traumatic Brain Injury.
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严重创伤性脑损伤后肠道细菌的易位和传播
DOI：
10.3390/microorganisms10102082
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发表时间：
2022-10-21
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
肠杆菌科细菌常见于重型颅脑损伤患者的肺部。然而，目前还不清楚这些细菌是否来自肠道微生物区系。为了验证这一假说，本研究采用了sTBI小鼠模型。STBI后，肺组织Chao1和Simpson指数在伤后7d达高峰(p<；0.05)。伤后7d，肺内不动杆菌的相对丰度增加到16.26%。肠道微生物区系的Chao1指数在伤后7d达到高峰(p<；0.05)。伤后3h，条件致病菌如沙眼衣原体、不动杆菌、类杆菌和链球菌明显增多。伤后7d和14d，sTBI组的组织学评分明显高于对照组(p<；0.05)。髓过氧化物酶(MPO)活性在伤后各时间点均升高，7d达高峰(p<；0.05)。LBP和sCD14在伤后7d达高峰(p<0.05)。STBI后3d，Zonlin显著升高，并维持在较高水平(p<；0.05)。经SourceTracker鉴定，伤后7d肺组织微生物区系可反映49.69%的肠道来源。在小肠，sTBI导致胃肠功能障碍，细胞凋亡增加，抗菌肽减少。肠道条件致病菌与Paneth细胞抗菌肽表达水平呈负相关。我们的数据表明，sTBI后肠道细菌转移到肺部，Paneth细胞可能调节肠道微生物区系的稳定性和易位。
Enterobacteriaceae are often found in the lungs of patients with severe Traumatic Brain Injury (sTBI). However, it is unknown whether these bacteria come from the gut microbiota. To investigate this hypothesis, the mice model of sTBI was used in this study. After sTBI, Chao1 and Simpson index peaking at 7 d in the lungs (p < 0.05). The relative abundance of Acinetobacter in the lungs increased to 16.26% at 7 d after sTBI. The chao1 index of gut microbiota increased after sTBI and peaked at 7 d (p < 0.05). Three hours after sTBI, the conditional pathogens such as Lachnoclostridium, Acinetobacter, Bacteroides and Streptococcus grew significantly. At 7 d and 14 d, the histology scores in the sTBI group were significantly higher than the control group (p < 0.05). The myeloperoxidase (MPO) activity increased at all-time points after sTBI and peaked at 7 d (p < 0.05). The LBP and sCD14 peaking 7 d after sTBI (p < 0.05). The Zonulin increased significantly at 3 d after sTBI and maintained the high level (p < 0.05). SourceTracker identified that the lung tissue microbiota reflects 49.69% gut source at 7 d after sTBI. In the small intestine, sTBI induced gastrointestinal dysfunction with increased apoptosis and decreasing antimicrobial peptides. There was a negative correlation between gut conditional pathogens and the expression level of antimicrobial peptides in Paneth cells. Our data indicate that gut bacteria translocated to the lungs after sTBI, and Paneth cells may regulate gut microbiota stability and translocation.
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