Traffic generated emissions alter the lung microbiota by promoting the expansion of Proteobacteria in C57Bl/6 mice placed on a high-fat diet.
Traffic generated emissions alter the lung microbiota by promoting the expansion of Proteobacteria in C57Bl/6 mice placed on a high-fat diet.
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在高脂肪饮食的C57Bl/6小鼠中，交通产生的排放物通过促进变形菌群的扩张来改变肺部微生物群。
DOI：
10.1016/j.ecoenv.2021.112035
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发表时间：
2021-04-15
影响因子：
6.8
通讯作者：
Lund AK
中科院分区：
文献类型：
--
作者：
Daniel S;Pusadkar V;McDonald J;Mirpuri J;Azad RK;Goven A;Lund AK
空气污染已被证明会导致严重的呼吸道疾病，如哮喘和慢性阻塞性肺病（COPD）。虽然这些疾病证明了肺部微生物群向变形菌门的转变，但交通产生的排放对肺部微生物群分布的影响尚未得到很好的表征。因此，我们研究了暴露于交通产生的排放物可以改变肺部微生物群和免疫防御的假设。由于西方世界的大量人口消费富含脂肪的饮食，我们试图调查混合车辆排放和高脂肪饮食消费的协同效应。我们将3个月大的雄性C57 Bl/6小鼠置于常规食物（LF）或高脂肪（HF：45%kcal脂肪）饮食中，暴露于混合排放物（ME：30 μg PM/m3汽油发动机排放物+ 70 μg PM/m3柴油发动机排放物）或过滤空气（FA）中，6小时/天，7天/周，持续30天。通过ELISA分析肺免疫球蛋白伊加、IgG和IgM的水平，并使用qPCR和Illumina 16 S测序进行肺微生物谱分析。我们观察到肺伊加显着减少ME暴露的动物相比，FA暴露的动物，都喂HF饮食。我们的研究结果还显示，与FA暴露的动物相比，LF饮食和HF饮食的ME暴露的动物的肺IgG显著降低。我们还观察到扩大肠杆菌科属于变形菌门的ME暴露组的HF饮食。总的来说，我们发现ME和HF饮食的联合作用导致免疫监视降低和肺部细菌生态失调，这在肺部疾病中具有重要意义。
Air pollution has been documented to contribute to severe respiratory diseases like asthma and chronic obstructive pulmonary disorder (COPD). Although these diseases demonstrate a shift in the lung microbiota towards Proteobacteria, the effects of traffic generated emissions on lung microbiota profiles have not been well-characterized. Thus, we investigated the hypothesis that exposure to traffic-generated emissions can alter lung microbiota and immune defenses. Since a large population of the Western world consumes a diet rich in fats, we sought to investigate the synergistic effects of mixed vehicle emissions and high-fat diet consumption. We exposed 3-month-old male C57Bl/6 mice placed either on regular chow (LF) or a high-fat (HF: 45% kcal fat) diet to mixed emissions (ME: 30 μg PM/m3 gasoline engine emissions + 70 μg PM/m3 diesel engine emissions) or filtered air (FA) for 6 h/d, 7 d/wk for 30 days. Levels of pulmonary immunoglobulins IgA, IgG, and IgM were analyzed by ELISA, and lung microbial profiling was done using qPCR and Illumina 16 S sequencing. We observed a significant decrease in lung IgA in the ME-exposed animals, compared to the FA-exposed animals, both fed a HF diet. Our results also revealed a significant decrease in lung IgG in the ME-exposed animals both on the LF diet and HF diet, in comparison to the FA-exposed animals. We also observed an expansion of Enterobacteriaceae belonging to the Proteobacteria phylum in the ME-exposed groups on the HF diet. Collectively, we show that the combined effects of ME and HF diet result in decreased immune surveillance and lung bacterial dysbiosis, which is of significance in lung diseases.
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影响因子：
4
作者：
Mathieu E;Escribano-Vazquez U;Descamps D;Cherbuy C;Langella P;Riffault S;Remot A;Thomas M
通讯作者：
Thomas M
暴露于环境颗粒物会改变微生物组成并诱导大鼠肺部的免疫变化。
影响因子：
5.8
作者：
Li N;He F;Liao B;Zhou Y;Li B;Ran P
通讯作者：
Ran P
影响因子：
--
作者：
Kim D;Chen Z;Zhou LF;Huang SX
通讯作者：
Huang SX
影响因子：
18.2
作者：
Dickson RP;Erb-Downward JR;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
影响因子：
8
作者：
Huffnagle GB;Dickson RP;Lukacs NW
通讯作者：
Lukacs NW