Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis.
Regeneration of oral siphon pigment organs in the ascidian Ciona intestinalis.
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DOI：
10.1016/j.ydbio.2009.12.040
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发表时间：
2010-03-15
影响因子：
2.7
通讯作者：
Jeffery WR
中科院分区：
文献类型：
--
作者：
Auger H;Sasakura Y;Joly JS;Jeffery WR
海鞘具有强大的再生能力，但其潜在机制却知之甚少。在这里，我们研究口腔虹吸再生的孤独海鞘玻璃海鞘。截肢后，口腔虹吸管迅速改革口腔色素器官（OPO）在其远端边缘之前，近端虹吸管部分再生较慢。口腔虹吸管重建的早期阶段包括细胞增殖和虹吸神经的再生长，尽管神经复合体（成人脑和相关器官）不需要再生。年轻的动物改革OPO更迅速截肢后比老年动物表明再生是年龄依赖性的。紫外线照射，显微烧灼，培养的虹吸外植体实验表明，OPO被替换为独立的单位的基础上的祖细胞在虹吸管的局部分化，而不是从一个遥远的来源在体内的细胞迁移。8个OPO和虹吸叶的典型模式恢复与保真度后，远端切断口虹吸，但多达16个OPO和叶可以改造后，近端切断虹吸基地附近。因此，OPO再生的模式由沿着口腔虹吸管的近端远端轴沿着定位的线索确定。提出了一个模型，其中柱的虹吸组织沿着的近端-远端轴低于预先存在的OPO负责再生过程中的正常OPO模式。这项研究揭示了以前未知的原则，口腔虹吸和OPO再生，将是重要的发展玻璃海鞘作为再生模型在urochordates，这可能是最近的生活亲属的脊椎动物。
Ascidians have powerful capacities for regeneration but the underlying mechanisms are poorly understood. Here we examine oral siphon regeneration in the solitary ascidian Ciona intestinalis. Following amputation, the oral siphon rapidly reforms oral pigment organs (OPO) at its distal margin prior to slower regeneration of proximal siphon parts. The early stages of oral siphon reformation include cell proliferation and re-growth of the siphon nerves, although the neural complex (adult brain and associated organs) is not required for regeneration. Young animals reform OPO more rapidly after amputation than old animals indicating that regeneration is age dependent. UV irradiation, microcautery, and cultured siphon explant experiments indicate that OPOs are replaced as independent units based on local differentiation of progenitor cells within the siphon, rather than by cell migration from a distant source in the body. The typical pattern of eight OPOs and siphon lobes is restored with fidelity after distal amputation of the oral siphon, but as many as 16 OPOs and lobes can be reformed following proximal amputation near the siphon base. Thus, the pattern of OPO regeneration is determined by cues positioned along the proximal distal axis of the oral siphon. A model is presented in which columns of siphon tissue along the proximal–distal axis below pre-existing OPO are responsible for reproducing the normal OPO pattern during regeneration. This study reveals previously unknown principles of oral siphon and OPO regeneration that will be important for developing Ciona as a regeneration model in urochordates, which may be the closest living relatives of vertebrates.
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影响因子：
0.8
作者：
Girvan, JE;Olson, WM;Hall, BK
通讯作者：
Hall, BK
影响因子：
2.7
作者：
Echeverri, K;Tanaka, EM
通讯作者：
Tanaka, EM
影响因子：
0.9
作者：
Chiba, S;Sasaki, A;Satoh, N
通讯作者：
Satoh, N
影响因子：
64.8
作者：
Delsuc, F;Brinkmann, H;Philippe, H
通讯作者：
Philippe, H
DOI：
10.1126/science.1147710
发表时间：
2007-11-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kumar A;Godwin JW;Gates PB;Garza-Garcia AA;Brockes JP
通讯作者：
Brockes JP