Development of a multiplexed microfluidic platform for the automated cultivation of embryonic stem cells.
Development of a multiplexed microfluidic platform for the automated cultivation of embryonic stem cells.
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DOI：
10.1177/2211068213499917
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Szita N
中科院分区：
文献类型：
--
作者：
Reichen M;Veraitch FS;Szita N
我们提出了一个用于微加工干细胞培养装置的多重平台。该模块化平台包含以自动化方式控制干细胞培养条件的所有组件。它在灌注培养过程中不需要培养箱，可以安装在倒置荧光显微镜的载物台上对干细胞培养物进行高频成像。压力驱动泵提供对介质流量的控制并提供流量的切换。泵的流速针对不同的压力设置进行表征，并且显示了细胞培养装置中施加的压力和流速之间的线性相关性。此外，该泵与两个培养基储存器一起运行，从而能够在细胞培养实验期间即时切换培养基。此外，通过我们的平台，可以冷却培养基储存器，以防止长期实验过程中培养基降解。然后在进入微型细胞培养装置之前将培养基温度调节至较高的受控温度。此外，温度在微型培养装置本身中进行调节。使用小鼠胚胎干细胞进行了初步培养实验。
We present a multiplexed platform for a microfabricated stem cell culture device. The modular platform contains all the components to control stem cell culture conditions in an automated fashion. It does not require an incubator during perfusion culture and can be mounted on the stage of an inverted fluorescence microscope for high-frequency imaging of stem cell cultures. A pressure-driven pump provides control over the medium flow rate and offers switching of the flow rates. Flow rates of the pump are characterized for different pressure settings, and a linear correlation between the applied pressure and the flow rate in the cell culture devices is shown. In addition, the pump operates with two culture medium reservoirs, thus enabling the switching of the culture medium on-the-fly during a cell culture experiment. Also, with our platform, the culture medium reservoirs are cooled to prevent medium degradation during long-term experiments. Media temperature is then adjusted to a higher controlled temperature before entering the microfabricated cell culture device. Furthermore, the temperature is regulated in the microfabricated culture devices themselves. Preliminary culture experiments are demonstrated using mouse embryonic stem cells.
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影响因子：
2.7
作者：
Petronis, S;Stangegaard, M;Dufva, M
通讯作者：
Dufva, M
DOI：
10.1126/science.1171643
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Discher DE;Mooney DJ;Zandstra PW
通讯作者：
Zandstra PW
影响因子：
3.7
作者：
Reichen M;Macown RJ;Jaccard N;Super A;Ruban L;Griffin LD;Veraitch FS;Szita N
通讯作者：
Szita N
影响因子：
56.9
作者：
Thomson, JA;Itskovitz-Eldor, J;Jones, JM
通讯作者：
Jones, JM
影响因子：
--
作者：
Mason, Chris;Hoare, Mike
通讯作者：
Hoare, Mike