Time-course effect of electrical stimulation on nerve regeneration of diabetic rats.
Time-course effect of electrical stimulation on nerve regeneration of diabetic rats.
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DOI：
10.1371/journal.pone.0116711
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chen YS
中科院分区：
文献类型：
--
作者：
Lin YC;Kao CH;Chen CC;Ke CJ;Yao CH;Chen YS
电刺激（ES）已被证明可以促进使用链脲佐菌素（STZ）诱导的实验性糖尿病大鼠的神经再生。然而，ES对糖尿病动物神经再生的时程效应在以前的研究中尚未报道。本研究旨在探讨糖尿病大鼠周围神经切断后不同时间ES的作用。本研究中使用了50只Sprague-Dawley大鼠。他们被分为五组。A ~ D组建立STZ糖尿病模型。E组为正常对照组。在所有组中，坐骨神经被切断并使用硅橡胶导管穿过10 mm的间隙进行修复。A ~ C组隔日1次，每次15 min，共2周。A组在神经修复后第1天开始刺激，B组在神经修复后第8天开始刺激，C组在神经修复后第15天开始刺激。糖尿病对照组D和正常对照组E不给予ES。术后30 d，A组10 mm神经缺损的再生成功率明显高于D组，电生理结果显示，与D组相比，A组再神经支配的腓肠肌的诱发肌肉动作电位面积和振幅的平均值明显增大。结论：ES的立即发作可以改善糖尿病动物大神经缺损的功能恢复。
Electrical stimulation (ES) has been shown to promote nerve regeneration in rats with experimental diabetes induced using streptozotocin (STZ). However, the time-course effect of ES on nerve regeneration of diabetic animals has not been reported in previous studies. The present study attempted to examine the effect of different timing of ES after peripheral nerve transection in diabetic rats. Fifty Sprague-Dawley rats were used in the study. They were classified into five groups. STZ-induced diabetes was created in groups A to D. Normal animals in group E were used as the non-diabetic controls. The sciatic nerve was transected and repaired using a silicone rubber conduit across a 10-mm gap in all groups. Groups A to C received ES for 15 minutes every other day for 2 weeks. Stimulation was initiated on day 1 following the nerve repair for group A, day 8 for group B, and day 15 for group C. The diabetic control group D and the normal control group E received no ES. At 30 days after surgery in group A, histological evaluations showed a higher success percentage of regeneration across the 10-mm nerve gap, and the electrophysiological results showed significantly larger mean values of evoked muscle action potential area and amplitude of the reinnervated gastrocnemius muscle compared with group D. It is concluded that an immediate onset of ES may improve the functional recovery of large nerve defect in diabetic animals.
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DOI：
10.1097/nen.0b013e31817e23f4
发表时间：
2008-07
影响因子：
3.2
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DOI：
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发表时间：
2009-11-01
影响因子：
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