Scatter-plate microscope: improved image acquisition

Scatter-plate microscope: improved image acquisition
复制标题

散射板显微镜:改进的图像采集

DOI:
10.1117/12.2306252
复制
发表时间:
2018
期刊:
影响因子:
--
通讯作者:
Wolfgang Osten
Wolfgang Osten
中科院分区:
--
文献类型:
--
作者:
Stephan Ludwig;Wolfgang Osten

文献摘要

参考文献

相似文献

我们研究如何提高图像分辨率和对比度的散射板显微镜图像去卷积和散斑图案的操作。散射板显微镜使用一个单一的扩散散射元件,而不是一个复杂的透镜系统,以记录几乎任意放大倍率的高分辨率图像。通过将点光源的散斑图案与非相干照明样品的散斑图案互相关来获取样品的图像。其工作原理受到光学记忆效应的有限范围和用于近似点源的光源(在我们的情况下为单模光纤)的质量的限制。利用点源散斑图案的自相关性作为滤波函数来描述采集图像与原始物体之间的关系的反卷积方法,可以部分补偿所用点源与理想点源的偏差。通过对散斑图的处理,可以减小记忆效应的有限范围的影响。
We investigate ways to improve image resolution and contrast in scatter-plate microscopy by image deconvolution and speckle pattern manipulation. Scatter-plate microscopy uses a single diffusively scattering element instead of a complex lens system to record high resolution images with almost arbitrary magnification. The image of the sample is acquired by cross-correlating the speckle pattern of a point source and the speckle pattern of the incoherently illuminated sample. The working principle is restricted by the finite range of the optical memory effect and by the quality of the light source used to approximate a point source (in our case a single mode fibre). With a deconvolution method using the autocorrelation of the point source speckle pattern as the filter function, describing the relationship between the acquired image and the original object, it is possible to compensate partially the deviation of the used point source from an ideal one. The influence of the restricted range of the memory effect can be reduced by manipulating the sample’s speckle pattern.
DOI: 10.1038/s41598-017-10767-3
发表时间: 2017-09-06
期刊: Scientific reports
影响因子: 4.6
作者:
Singh AK;Pedrini G;Takeda M;Osten W
通讯作者: Osten W
DOI: --
发表时间: 2018
期刊: BiOS
影响因子: --
作者:
W. Osten;K. Frenner;G. Pedrini;A. Singh;J. Schindler;M. Takeda
通讯作者: M. Takeda