The astonishing diversity of Ig classes and B cell repertoires in teleost fish.
The astonishing diversity of Ig classes and B cell repertoires in teleost fish.
复制标题
DOI：
10.3389/fimmu.2013.00028
复制
发表时间：
2013
影响因子：
7.3
通讯作者：
Boudinot P
中科院分区：
文献类型：
--
作者：
Fillatreau S;Six A;Magadan S;Castro R;Sunyer JO;Boudinot P
与哺乳动物不同的淋巴组织解剖学，以及对所有水生环境的不同适应，鱼类构成了一个迷人的脊椎动物群体，以比较的角度研究B细胞库的生物学。鱼类B淋巴细胞在其表面上表达免疫球蛋白（IG）并分泌抗原特异性抗体以响应免疫挑战。在鱼类中已经鉴定出三种抗体类别，即IgM、IgD和IgT，而IgG、IgA和IgE不存在。IgM和IgD已被发现在所有鱼类物种分析，因此似乎是原始抗体类。IgM和IgD通常通过选择性剪接从相同的mRNA共表达，如在哺乳动物中。四聚体IgM是血清中发现的主要抗体类别。有些鱼类也有IgT，似乎只存在于鱼类中，专门用于粘膜免疫。IgM/IgD和IgT由两种不同的B细胞亚群表达。在鱼类细胞水平上研究B细胞反应的工具是有限的，但鱼类基因组学的进展已经开始揭示丰富多样的IgH和免疫球蛋白轻链位点组织，这可能与鱼类进化过程中发生的基因组重塑的继承。此外，深度测序技术的发展已经允许研究斑马鱼和虹鳟鱼中表达的鱼B细胞库在稳定状态下或感染后的全局特征。本文综述了鱼类免疫球蛋白IG基因座的组织结构，特别强调其物种间的异质性，并提出了最近的数据在健康和感染的鱼表达的IG库的结构。
With lymphoid tissue anatomy different than mammals, and diverse adaptations to all aquatic environments, fish constitute a fascinating group of vertebrate to study the biology of B cell repertoires in a comparative perspective. Fish B lymphocytes express immunoglobulin (Ig) on their surface and secrete antigen-specific antibodies in response to immune challenges. Three antibody classes have been identified in fish, namely IgM, IgD, and IgT, while IgG, IgA, and IgE are absent. IgM and IgD have been found in all fish species analyzed, and thus seem to be primordial antibody classes. IgM and IgD are normally co-expressed from the same mRNA through alternative splicing, as in mammals. Tetrameric IgM is the main antibody class found in serum. Some species of fish also have IgT, which seems to exist only in fish and is specialized in mucosal immunity. IgM/IgD and IgT are expressed by two different sub-populations of B cells. The tools available to investigate B cell responses at the cellular level in fish are limited, but the progress of fish genomics has started to unravel a rich diversity of IgH and immunoglobulin light chain locus organization, which might be related to the succession of genome remodelings that occurred during fish evolution. Moreover, the development of deep sequencing techniques has allowed the investigation of the global features of the expressed fish B cell repertoires in zebrafish and rainbow trout, in steady state or after infection. This review provides a description of the organization of fish Ig loci, with a particular emphasis on their heterogeneity between species, and presents recent data on the structure of the expressed Ig repertoire in healthy and infected fish.
登录
查看更多内容
影响因子：
4.8
作者：
Fisher, RA;Corbet, AS;Williams, CB
通讯作者：
Williams, CB
DOI：
10.1038/nrg2703
发表时间：
2010-01
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Edholm, Eva-Stina;Bengten, Eva;Wilson, Melanie
通讯作者：
Wilson, Melanie
影响因子：
3.2
作者：
Edholm, Eva-Stina;Wilson, Melanie;Bengten, Eva
通讯作者：
Bengten, Eva
影响因子：
2.5
作者：
Bladen, Catherine L.;Navarre, Sammy;Kozlowski, David J.
通讯作者：
Kozlowski, David J.