Effects of bone‐seeking hormones on DNA synthesis, cyclic AMP level, and alkaline phosphatase activity in cultured cells from human posterior longitudinal ligament of the spine
Effects of bone‐seeking hormones on DNA synthesis, cyclic AMP level, and alkaline phosphatase activity in cultured cells from human posterior longitudinal ligament of the spine
复制标题
寻骨激素对人脊柱后纵韧带培养细胞DNA合成、环磷酸腺苷水平和碱性磷酸酶活性的影响
DOI：
10.1002/jbmr.5650081104
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发表时间：
1993
影响因子：
6.2
通讯作者：
Shinya Kawai
中科院分区：
文献类型：
--
作者：
Y. Ishida;Shinya Kawai
在人脊柱后纵韧带成骨能力的研究中，分离并培养韧带细胞。研究了趋骨激素（如甲状旁腺激素（PTH）、降钙素（CT）、前列腺素E2（PGE 2）和1，25-二羟基胆钙化醇[1，25-（OH）2D 3]）对韧带细胞DNA合成、3′，5 ′-环磷酸腺苷（cAMP）水平、碱性磷酸酶（ALP）活性和酸性磷酸酶（ACP）活性的影响。从脊柱后纵韧带骨化（OPLL）患者的非骨化部位获得的细胞系被发现具有成骨细胞的几种不同表型特征：高ALP活性，PTH-和PGE 2刺激的cAMP增加，以及对CT和1，25-（OH）2D 3的反应。很明显，这些韧带细胞的增殖和分化受到各种类型的趋骨激素的控制，并且表明存在许多具有成骨细胞样特征的细胞。这些结果被认为是重要的OPLL的病因学方面，使用培养的韧带细胞的实验系统似乎是有用的OPLL的研究。
In a study of the osteogenesis capability of the human posterior longitudinal ligament of the spine, ligament cells were isolated and cultured. The effect of bone‐seeking hormones, such as parathyroid hormone (PTH), calcitonin (CT), prostaglandin E2 (PGE2), and 1,25‐dihydroxycholecalciferol [1,25‐(OH)2D3], on the ligament cells was investigated with respect to DNA synthesis, adenosine‐3′,5′‐cyclic monophosphate (cAMP) levels, alkaline phosphatase (ALP) activity, and acid phosphatase (ACP) activity. Cell lines obtained from nonossified sites in patients with ossification of the posterior longitudinal ligament of the spine (OPLL) were found to have several different phenotypic characteristics for osteoblasts: high ALP activity, PTH‐ and PGE2‐stimulated increases in cAMP, and responses to both CT and 1,25‐(OH)2D3. It is clear that proliferation and differentiation in such ligament cells are controlled by various types of bone‐seeking hormones, and it was suggested that many cells with osteoblast‐like characteristics are present. These results are considered important with respect to the etiology of OPLL, and an experimental system using cultured ligament cells appears to be useful in research on OPLL.
影响因子：
9.8
作者：
CANALIS, E
通讯作者：
CANALIS, E
1,25-二羟基维生素 D3 可增加人成骨肉瘤细胞中骨碱性磷酸酶同工酶的水平。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mulkins,MA;Manolagas,SC;Deftos,LJ;Sussman,HH
通讯作者：
Sussman,HH
甲状旁腺激素受体的光亲和标记：跨物种和靶组织以及激素脱敏后受体的比较。
影响因子：
2.9
作者：
Goldring,SR;Tyler,GA;Krane,SM;PottsJr,JT;Rosenblatt,M
通讯作者：
Rosenblatt,M