Photoaffinity labeling of parathyroid hormone receptors: comparison of receptors across species and target tissues and after desensitization to hormone.
Photoaffinity labeling of parathyroid hormone receptors: comparison of receptors across species and target tissues and after desensitization to hormone.
复制标题
甲状旁腺激素受体的光亲和标记：跨物种和靶组织以及激素脱敏后受体的比较。
DOI：
10.1021/bi00298a015
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Rosenblatt,M
中科院分区：
文献类型：
--
作者：
Goldring,SR;Tyler,GA;Krane,SM;PottsJr,JT;Rosenblatt,M
Steven R. Goldring，** 乔治A.放大图片作者：泰勒约翰·克兰小波茨，Michael Rosenblatt摘要：来源于人骨巨细胞瘤的细胞和来源于人新生儿包皮的成纤维细胞通过增加胞内和胞外腺苷环磷酸（cAMP）水平对甲状旁腺激素（PTH）作出反应。使用光亲和素标记方法，我们检查了这些细胞的PTH受体或受体复合物的结合亚基的存在。一个以前设计的生物活性和photolabileradioligand类似物的PTH与这些完整的细胞反应。光解后，提取细胞，蛋白质变性、还原，并通过十二烷基硫酸钠（Na-DodS 04）-聚丙烯酰胺凝胶电泳分离，然后进行放射自显影。一个单一的膜组件，Mr 70 000，标记特异性在骨骼和真皮组织培养的完整细胞。通过成对混合来自（a）来源于骨巨细胞瘤的完整人细胞、（B）完整人成纤维细胞和（c）犬肾皮质膜的光标记蛋白质，受体（或其结合亚基）的PTH直接相同，并发现在分子大小方面是相同的（通过NaDod-S 04-聚丙烯酰胺凝胶上的迁移位置确定）跨种属（犬和人）和靶组织（骨、皮肤和肾脏）。从骨巨细胞瘤中培养的细胞与PTH预孵育导致PTH诱导的cAMP反应丧失（脱敏）。用PTH预孵育伴随着PTH结合组分的光亲和素标记的显著减少，并表明用PTH预孵育的细胞中激素反应的丧失与PTH受体的数量或可用性的减少有关。与此相反，PTH诱导的cAMP反应和光亲和标记的PTH结合成分没有改变通过预孵育细胞与PTH抑制剂结合PTH受体，但未能增加cAMP水平。这些结果表明，简单
Steven R. Goldring,** George A. Tyler, Stephen M. Krane, John T. Potts, Jr., and Michael Rosenblatt abstract: Cells derived from human giantcell tumors of bone and fibroblasts derived from humanneonatal foreskin respond to parathyroid hormone (PTH) by increasing the intracellular and extracellular levels of adenosine cyclic 3', 5'-phosphate (cAMP). Using photoaffmity labeling methods, we examined these cells for the presence of a PTH receptor or a binding subunit of a receptor complex. A previously designed biologically activeand photolabileradioligand analogue of PTH was reacted with these intact cells. After photolysis, the cells were extracted, and the proteins were denatured, reduced, and separated by electrophoresis on sodium dodecyl sulfate (Na-DodS04)-polyacrylamide gels followed by autoradiography. A single membrane component, Mr 70 000, was labeled spe-cifically in intact cells cultured from skeletal and dermal tissue. By mixing, in pairs, photolabeled proteins from (a) intact human cells derived from giant cell tumors of bone,(b) intact human fibroblasts, and (c) canine renal cortical membranes, the receptors (ortheir binding subunits) for PTH were com-pared directly and found to be identical in terms of molecular size (as determined by the migration position on NaDod-S04-polyacrylamide gels) across species (dog andhuman) and target tissue (bone, skin, and kidney). Preincubation of cells cultured from giant cell tumors of bone with PTH resulted in loss of the PTH-induced cAMP response (desensitization). Preincubation with PTH was accompanied by a marked de-crease in photoaffmity labeling of the PTH binding component and suggests that the loss of hormone response in cells preincubated with PTH was related to a decrease in the number or availability of PTH receptors. In contrast, the PTH-induced cAMP response and photoaffinity labeling of the PTH binding component were not altered by preincubating cells with a PTH inhibitor which binds to PTH receptors but fails to increase cAMP levels. These results suggest that simple
登录
查看更多内容
甲状旁腺激素抑制剂：骨源性组织和皮肤源性组织中生物活性的比较。
影响因子：
5.8
作者：
S. Goldring;J. Mahaffey;M. Rosenblatt;J. Dayer;J. Potts;S. Krane
通讯作者：
S. Krane
含有 D-氨基酸的无硫甲状旁腺激素类似物：体外和体内生物学特性。
影响因子：
2.9
作者：
Rosenblatt,M;Coltrera,MD;Shepard,GL;Gray,DA;Parsons,JA;PottsJr,JT
通讯作者：
PottsJr,JT
影响因子：
64.8
作者：
HUNTER, WM;GREENWOOD, FC
通讯作者：
GREENWOOD, FC
甲状旁腺激素抑制剂。
DOI：
10.1016/s0021-9258(17)40100-1
发表时间：
1977
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Rosenblatt;E. Callahan;J. Mahaffey;A. Pont;J. Potts
通讯作者：
J. Potts
对从人类骨巨细胞瘤培养的细胞的激素的反应。
影响因子：
5.8
作者：
S. Goldring;J. Dayer;R. Russell;H. Mankin;S. Krane
通讯作者：
S. Krane