Nodule formation in Bombyx mori larvae is regulated by BmToll10-3.
Nodule formation in Bombyx mori larvae is regulated by BmToll10-3.
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家蚕幼虫中的结节形成受 BmToll10-3 调节。
DOI：
10.1016/j.jinsphys.2022.104441
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Sato R.
中科院分区：
文献类型：
--
作者：
Suzuki T;Tang S;Otuka H;Ito K;Sato R.
免疫球蛋白形成是由细胞因子spätzle1引起的两步细胞介导的免疫应答。Spätzle1在微生物入侵后30 s内通过由病原体相关分子模式识别受体、C型凝集素和丝氨酸蛋白酶组成的细胞外信号传导途径被激活。在这里，我们研究了一种血细胞分子，参与引发结节形成的第一步。BmToll 10 - 3是家蚕中鉴定的14个Toll同源物之一，它是贪夜蛾Toll的直向同源物。以前的研究表明，SeToll诱发结节形成，但没有证据表明是否SeToll诱发结节形成的第一或第二步。逆转录-聚合酶链反应和免疫染色证实BmToll 10 - 3在粒细胞中表达。为了确定BmToll 10 - 3是否参与引发结节形成的第一步，我们在结节形成测定中测试了针对BmToll 10 - 3产生的抗血清。该抗血清强烈抑制了B.morilarvae根瘤形成的第一步。接下来，我们尝试使用基因组编辑敲除BmToll 10 - 3。产生了对于截短的BmToll 10 - 3等位基因为杂合的菌株，但没有产生对于截短的BmToll 10 - 3为纯合的菌株。尽管如此，几个健康的纯合子幼虫化蛹前，我们使用这些幼虫在结节形成试验。对于截短的BmToll 10 - 3纯合的幼虫不形成结节。这些结果表明BmToll 10 - 3参与细胞免疫，结节形成。
Nodule formation is a two-step cell-mediated immune response that is elicited by the cytokine spätzle1. Spätzle1 is activated within 30 s of invasion by microorganisms via an extracellular signaling pathway that consists of pathogen-associated molecular pattern recognition receptors, C-type lectins, and serine proteases. Here, we investigated a hemocyte molecule that is involved in eliciting the first step of nodule formation. BmToll10-3 was one of 14 Toll homologs identified in the silkwormBombyx mori; it is an ortholog ofSpodoptera exiguaToll. Previous research suggested that SeToll elicits nodule formation, but no evidence was presented to indicate whether SeToll elicited the first or second step of nodule formation. Reverse transcription-polymerase chain reaction and immunostaining confirmed that BmToll10-3 is expressed in granulocytes. To determine whether BmToll10-3 is involved in eliciting the first step of nodule formation, we tested an antiserum raised against BmToll10-3 in a nodule formation assay. The antiserum strongly inhibited the first step of nodule formation inB.morilarvae. Next, we tried to knock out BmToll10-3 using genome editing. Strains that were heterozygous for a truncated BmToll10-3 allele were generated, but no strain that was homozygous for truncated BmToll10-3 was generated. Nonetheless, several healthy homozygous larvae were identified before pupation, and we used these larvae in a nodule formation assay. The larvae that were homozygous for truncated BmToll10-3 did not form nodules. These results suggest that BmToll10-3 is involved in a cellular immunity, nodule formation.
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DOI：
10.1073/pnas.2103021118
发表时间：
2021-05
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Ruonan Zhang;Xiaofeng Li;Jie Zhang;Yanjun Li;Yuan Wang;Yuhang Song;Feifei Ren;Huiyu Yi;Xiaojuan Deng;Yangjin Zhong;Yang Cao;M. Strand;Xiao-qiang Yu;Wanying Yang
通讯作者：
Ruonan Zhang;Xiaofeng Li;Jie Zhang;Yanjun Li;Yuan Wang;Yuhang Song;Feifei Ren;Huiyu Yi;Xiaojuan Deng;Yangjin Zhong;Yang Cao;M. Strand;Xiao-qiang Yu;Wanying Yang
昆虫血细胞：血细胞在防御生物制剂中的作用
DOI：
--
发表时间：
1979
期刊：
影响因子：
--
作者：
N. Ratcliffe;A. Rowley
通讯作者：
A. Rowley
各种类二十烷酸调节甜菜夜蛾的细胞和体液免疫反应
DOI：
--
发表时间：
2009
期刊：
Bioscience, biotechnology and biochemistry
影响因子：
--
作者：
S. Shrestha;Yonggyun Kim
通讯作者：
Yonggyun Kim
调节家蚕血细胞结节形成的胞外和胞内信号通路（鳞翅目：Bombicidae）
影响因子：
--
作者：
Asahi Suzuki;Yasutaka Yoshizawa;Shiho Tanaka;Madoka Kitami;R. Sato
通讯作者：
R. Sato
影响因子：
5.3
作者：
Kanost MR;Jiang H
通讯作者：
Jiang H