The Two-Component Locus MSMEG_0244/0246 Together With MSMEG_0243 Affects Biofilm Assembly in M. smegmatis Correlating With Changes in Phosphatidylinositol Mannosides Acylation.
The Two-Component Locus MSMEG_0244/0246 Together With MSMEG_0243 Affects Biofilm Assembly in M. smegmatis Correlating With Changes in Phosphatidylinositol Mannosides Acylation.
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DOI：
10.3389/fmicb.2020.570606
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发表时间：
2020
影响因子：
5.2
通讯作者：
Jessen-Trefzer C
中科院分区：
文献类型：
--
作者：
Li M;Gašparovič H;Weng X;Chen S;Korduláková J;Jessen-Trefzer C
铁和亚铁是许多细菌物种包括分枝杆菌生长所必需的过渡金属。来自耻垢分枝杆菌的基因组区域msmeg_0234至msmeg_0252参与铁/血红素代谢。我们研究了编码假定的双组分系统MSMEG_0244/MSMEG_0246的基因、邻近基因msmeg_0243以及它们在该过程中的参与。我们表明，纯化的MSMEG_0243确实是一个血红素结合蛋白。在耻垢分枝杆菌中缺失msmeg_0243/msmeg_0244/msmeg_0246导致生物膜形成和固体琼脂上的菌落生长缺陷，然而，这种表型与所提供的铁源无关。此外，相应的突变体及其脂质的分析表明，形态和生物膜形成的变化与磷脂酰肌醇甘露糖苷（PIM）的酰化模式改变相关。我们提供的第一个证据表明，msmeg_0244/msmeg_0246协同工作，在细胞脂质稳态，特别是在维护PIM，与血红素结合蛋白MSMEG_0243作为潜在的合作伙伴。
Ferric and ferrous iron is an essential transition metal for growth of many bacterial species including mycobacteria. The genomic region msmeg_0234 to msmeg_0252 from Mycobacterium smegmatis is putatively involved in iron/heme metabolism. We investigate the genes encoding the presumed two component system MSMEG_0244/MSMEG_0246, the neighboring gene msmeg_0243 and their involvement in this process. We show that purified MSMEG_0243 indeed is a heme binding protein. Deletion of msmeg_0243/msmeg_0244/msmeg_0246 in Mycobacterium smegmatis leads to a defect in biofilm formation and colony growth on solid agar, however, this phenotype is independent of the supplied iron source. Further, analysis of the corresponding mutant and its lipids reveals that changes in morphology and biofilm formation correlate with altered acylation patterns of phosphatidylinositol mannosides (PIMs). We provide the first evidence that msmeg_0244/msmeg_0246 work in concert in cellular lipid homeostasis, especially in the maintenance of PIMs, with the heme-binding protein MSMEG_0243 as potential partner.
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影响因子：
5.4
作者：
Jackson, Mary
通讯作者：
Jackson, Mary
影响因子：
4.8
作者：
Korduláková, J;Gilleron, M;Jackson, M
通讯作者：
Jackson, M
影响因子：
4.8
作者：
Korduláková, J;Gilleron, M;Jackson, M
通讯作者：
Jackson, M
影响因子：
3.2
作者：
Boldrin, Francesca;Ventura, Marcello;Manganelli, Riccardo
通讯作者：
Manganelli, Riccardo
影响因子：
2.8
作者：
DHARIWAL, KR;CHANDER, A;VENKITASUBRAMANIAN, TA
通讯作者：
VENKITASUBRAMANIAN, TA