Biocompatible polysiloxane-containing diblock copolymer PEO-b-PgammaMPS for coating magnetic nanoparticles.
Biocompatible polysiloxane-containing diblock copolymer PEO-b-PgammaMPS for coating magnetic nanoparticles.
复制标题
DOI：
10.1021/am900262j
复制
发表时间：
2009-10
影响因子：
9.5
通讯作者：
Mao, Hui
中科院分区：
文献类型：
--
作者：
Chen, Hongwei;Wu, Xinying;Duan, Hongwei;Wang, Y. Andrew;Wang, Liya;Zhang, Minming;Mao, Hui
我们报道了一种含有两亲性两嵌段共聚物的生物相容聚硅氧烷，聚(乙烯oxide)-block-poly(γ-methacryloxypropyltrimethoxysilane))(PEO-b-PγMPS)，用于包覆和稳定生物医学应用的纳米颗粒。用PEO大分子链转移剂进行可逆加成断裂链转移(RAFT)聚合，得到了既含有“表面锚定基团”(硅烷基)的疏水链段和含有PEO(Mn=5000 g/mol)的亲水链段的两亲性两嵌段共聚物。将共聚物包覆顺磁性纳米氧化铁(IONPs)时，在四氢呋喃共溶剂中将疏水油酸包覆的粒径均匀的IONPs(D=13 nm)与共聚物混合。经过一段时间的老化后，得到的单分散IONPs可以转移到水介质中。在适当的P-γ-MPS嵌段长度下(Mn=10,000 g/m ol)，两嵌段共聚硅氧烷在单晶纳米粒子周围形成了一层薄薄的包覆层(~3 nm)。磁共振成像实验表明，包覆的IONPs具有良好的T2加权对比效果，其横向弛豫系数R2=98.6 mm−1s−1(1.5Tesla)。与传统的两亲性共聚物包覆的IONPs相比，这种薄的包覆层对IONPs的驰豫性能影响不大。含两嵌段共聚物的聚硅氧烷包裹IONPs在含10%血清的培养液中孵育24 h后，稳定性良好，不与血清中的蛋白质发生聚集或结合。此外，含有两嵌段共聚物的聚硅氧烷包裹IONPs后，巨噬细胞的细胞内摄取水平大大降低，这表明非特异性细胞摄取减少和抗生物污垢效应。
We report a biocompatible polysiloxane containing amphiphilic diblock copolymer, poly(ethylene oxide)-block-poly(γ-methacryloxypropyltrimethoxysilane) (PEO-b-PγMPS), for coating and stabilizing nanoparticles for biomedical applications. Such amphiphilic diblock copolymer which comprises both a hydrophobic segment with “surface anchoring moiety” (silane group) and a hydrophilic segment with PEO (Mn=5000 g/mol) was obtained by the reversible addition fragmentation chain transfer (RAFT) polymerization using the PEO macromolecular chain transfer agent. When used for coating paramagnetic iron oxide nanoparticles (IONPs), copolymers were mixed with hydrophobic oleic acid coated core size uniformed IONPs (D=13 nm) in co-solvent tetrahydrofuran. After being aged over a period of time, resulting monodispersed IONPs can be transferred into aqueous medium. With proper PγMPS block length (Mn=10,000 g/mol), polysiloxane containing diblock copolymers formed a thin layer of coating (~3 nm) around monocrystalline nanoparticles as measured by transmission electron microscopy (TEM). Magnetic resonance imaging (MRI) experiments showed excellent T2 weighted contrast effect from coated IONPs with a transverse relaxivity r2=98.6 mM−1s−1 (at 1.5 Tesla). Such thin coating layer has little effect on the relaxivity when compared to that of IONPs coated with conventional amphiphilic copolymer. Polysiloxane containing diblock copolymer coated IONPs are stable without aggregation or binding to proteins in serum when incubated for 24 h in culture medium containing 10% serum. Furthermore, much lower level of intracellular uptake by macrophage cells was observed with polysiloxane containing diblock copolymers coated IONPs, suggesting the reduction of non-specific cell uptakes and antibiofouling effect.
登录
查看更多内容
影响因子：
15
作者：
Lee, Haerim;Yu, Mi Kyung;Jon, Sangyong
通讯作者：
Jon, Sangyong
影响因子：
10.8
作者：
Nasongkla, Norased;Bey, Erik;Gao, Jinming
通讯作者：
Gao, Jinming
影响因子：
5.5
作者：
Mellon, W;Rinaldi, D;D'Agosto, F
通讯作者：
D'Agosto, F
影响因子：
10.8
作者：
Kim, BS;Qiu, JM;Taton, TA
通讯作者：
Taton, TA
影响因子：
3.9
作者：
Gindy, Marian E.;Panagiotopoulos, Athanassios Z.;Prud'homme, Robert K.
通讯作者：
Prud'homme, Robert K.