Chloroplast nucleoids as a transformable network revealed by live imaging with a microfluidic device.
Chloroplast nucleoids as a transformable network revealed by live imaging with a microfluidic device.
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DOI：
10.1038/s42003-018-0055-1
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发表时间：
2018
影响因子：
5.9
通讯作者：
Nishimura Y
中科院分区：
文献类型：
--
作者：
Kamimura Y;Tanaka H;Kobayashi Y;Shikanai T;Nishimura Y
叶绿体DNA被组织成DNA -蛋白质聚合体，称为叶绿体类核，它们被复制、转录和遗传。应用微流控装置实时成像技术对莱茵衣藻叶绿体类核性质进行了研究。在叶绿体分裂过程中，我们观察到球状叶绿体类核动态地、可逆地分散成网状结构。在单核叶绿体（moc）突变体中，由于MOC1的缺陷，叶绿体类核在叶绿体分裂后分布不均匀，叶绿体类核形成的早期阶段主要发生在近端区域。这表明叶绿体类核可转化网络由一个高度紧密的核心组成，其近端区域与cpDNA复制和类核形成有关。Yoshitaka Kamimura及其同事将实时成像技术与微流体技术结合起来，检测了类核中叶绿体DNA的组织。他们发现这些结构在叶绿体分裂过程中形成网状结构，并提出了它们在细胞器复制过程中的遗传机制。
Chloroplast DNA is organized into DNA–protein conglomerates called chloroplast nucleoids, which are replicated, transcribed, and inherited. We applied live-imaging technology with a microfluidic device to examine the nature of chloroplast nucleoids in Chlamydomonas reinhardtii. We observed the dynamic and reversible dispersion of globular chloroplast nucleoids into a network structure in dividing chloroplasts. In the monokaryotic chloroplast (moc) mutant, in which chloroplast nucleoids are unequally distributed following chloroplast division due to a defect in MOC1, the early stages of chloroplast nucleoid formation occurred mainly in the proximal area. This suggests the chloroplast nucleoid transformable network consists of a highly compact core with proximal areas associated with cpDNA replication and nucleoid formation. Yoshitaka Kamimura and colleagues combine live-imaging technology with microfluidics to examine chloroplast DNA organization in nucleoids. They find that these structures form a network structure in dividing chloroplasts, and propose a mechanism for their inheritance in organelle replication.
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