The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion.
The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion.
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DOI：
10.1038/srep02723
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发表时间：
2013-10-14
影响因子：
4.6
通讯作者：
Matsunaga, Sachihiro
中科院分区：
文献类型：
--
作者：
Hayashi, Kohma;Hasegawa, Junko;Matsunaga, Sachihiro
植物根由有丝分裂细胞的分生区和快速扩展细胞的伸长区组成，其中DNA复制通常在没有细胞分裂的情况下发生，这一过程称为核内复制。用5-乙炔基-2 ′-脱氧尿苷（EdU）掺入法测定的细胞周期和DNA复制的持续时间在两个区域之间不同（分生区为17 h，延伸区为30 h）。 两种不同的亚核模式的EdU信号，整个和斑点，标记细胞核进行DNA复制在早期和晚期S期，分别。通过DNA复制成像和每个细胞核中DNA量的光学估计（C值）的组合来分析分生区和延伸区之间的边界区域。我们发现了一个边界细胞与4C核表现出整个模式的EdU信号。在边界区域的细胞分析表明，核内复制之前快速细胞伸长根。
Plant roots consist of a meristematic zone of mitotic cells and an elongation zone of rapidly expanding cells, in which DNA replication often occurs without cell division, a process known as endoreduplication. The duration of the cell cycle and DNA replication, as measured by 5-ethynyl-2′-deoxy-uridine (EdU) incorporation, differed between the two regions (17 h in the meristematic zone, 30 h in the elongation zone). Two distinct subnuclear patterns of EdU signals, whole and speckled, marked nuclei undergoing DNA replication at early and late S phase, respectively. The boundary region between the meristematic and elongation zones was analysed by a combination of DNA replication imaging and optical estimation of the amount of DNA in each nucleus (C-value). We found a boundary cell with 4C nuclei exhibiting the whole pattern of EdU signals. Analyses of cells in the boundary region revealed that endoreduplication precedes rapid cell elongation in roots.
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