Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis.
Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis.
复制标题
DOI：
10.1083/jcb.200508001
复制
发表时间：
2006-01-02
期刊：
影响因子：
--
通讯作者：
Rudnicki MA
中科院分区：
文献类型：
--
作者：
Kuang S;Chargé SB;Seale P;Huh M;Rudnicki MA
我们评估了129Sv/J背景下存活的Pax7 - / -小鼠，观察到生长减少和明显的肌肉萎缩，同时完全没有功能性卫星细胞。急性损伤导致肌肉再生极度不足。然而，少量再生肌纤维被检测到，这表明在pax7缺乏的肌肉中存在残留的肌原细胞。罕见的Pax3+/MyoD+肌母细胞来自Pax7−/−肌肉匀浆和肌纤维束培养物，但没有来自无间质组织的单个肌纤维。最后，我们在肌肉间质环境中鉴定了Pax3+细胞，并证明它们在再生过程中共表达MyoD。后肢肌肉的层下卫星细胞未表达可检测水平的Pax3蛋白或信使RNA。因此，我们得出结论，间质Pax3+细胞代表了一种不同于层下卫星细胞谱系的新型肌源性细胞群，Pax7对于层下卫星细胞形成功能性肌源性祖细胞至关重要。
We assessed viable Pax7 − / − mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted in an extreme deficit in muscle regeneration. However, a small number of regenerated myofibers were detected, suggesting the presence of residual myogenic cells in Pax7-deficient muscle. Rare Pax3+/MyoD+ myoblasts were recovered from Pax7 − / − muscle homogenates and cultures of myofiber bundles but not from single myofibers free of interstitial tissues. Finally, we identified Pax3+ cells in the muscle interstitial environment and demonstrated that they coexpressed MyoD during regeneration. Sublaminar satellite cells in hind limb muscle did not express detectable levels of Pax3 protein or messenger RNA. Therefore, we conclude that interstitial Pax3+ cells represent a novel myogenic population that is distinct from the sublaminar satellite cell lineage and that Pax7 is essential for the formation of functional myogenic progenitors from sublaminar satellite cells.
登录
查看更多内容
DOI：
10.1083/jcb.200108150
发表时间：
2002-05-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Qu-Petersen Z;Deasy B;Jankowski R;Ikezawa M;Cummins J;Pruchnic R;Mytinger J;Cao B;Gates C;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
7.8
作者：
Asakura, Atsushi;Seale, Patrick;Girgis-Gabardo, Adele;Rudnicki, Michael A
通讯作者：
Rudnicki, Michael A
DOI：
10.1007/s004290050237
发表时间：
1999-05-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Bittner, RE;Schöfer, C;Wachtler, F
通讯作者：
Wachtler, F
影响因子：
10.5
作者：
Kassar-Duchossoy, L;Giacone, E;Tajbakhsh, S
通讯作者：
Tajbakhsh, S
影响因子：
2.7
作者：
Olguin, HC;Olwin, BB
通讯作者：
Olwin, BB