Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration.
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration.
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DOI：
10.1083/jcb.200108150
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发表时间：
2002-05-27
期刊：
影响因子：
--
通讯作者：
Huard J
中科院分区：
文献类型：
--
作者：
Qu-Petersen Z;Deasy B;Jankowski R;Ikezawa M;Cummins J;Pruchnic R;Mytinger J;Cao B;Gates C;Wernig A;Huard J
从正常小鼠骨骼肌中分离出三种肌源性细胞，并对其粘附特性和增殖特性进行了研究。虽然这些人口中的两个显示卫星细胞的特征，第三人口的长期增殖细胞表达造血干细胞标记物也被确定。这第三个群体包含的细胞保持其表型超过30代，具有正常核型，并可以在体外和体内分化成肌肉，神经和内皮谱系。与其他两个群体的肌源性细胞相比，长期增殖细胞的移植提高了肌肉再生和肌营养不良蛋白递送到营养不良肌肉的效率。长时间增殖的细胞在体内长时间增殖的能力，结合它们强大的自我更新能力、它们的多能分化和它们的免疫特权行为，至少部分地揭示了细胞移植改进的基础。我们的研究结果表明，这种新的肌源性干细胞群体将显着改善肌细胞介导的治疗。
Three populations of myogenic cells were isolated from normal mouse skeletal muscle based on their adhesion characteristics and proliferation behaviors. Although two of these populations displayed satellite cell characteristics, a third population of long-time proliferating cells expressing hematopoietic stem cell markers was also identified. This third population comprises cells that retain their phenotype for more than 30 passages with normal karyotype and can differentiate into muscle, neural, and endothelial lineages both in vitro and in vivo. In contrast to the other two populations of myogenic cells, the transplantation of the long-time proliferating cells improved the efficiency of muscle regeneration and dystrophin delivery to dystrophic muscle. The long-time proliferating cells' ability to proliferate in vivo for an extended period of time, combined with their strong capacity for self-renewal, their multipotent differentiation, and their immune-privileged behavior, reveals, at least in part, the basis for the improvement of cell transplantation. Our results suggest that this novel population of muscle-derived stem cells will significantly improve muscle cell–mediated therapies.
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DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
3.4
作者：
HUARD, J;BOUCHARD, JP;TREMBLAY, JP
通讯作者：
TREMBLAY, JP
影响因子：
20.3
作者：
FENNIE, C;CHENG, J;LASKY, LA
通讯作者：
LASKY, LA
影响因子：
3.3
作者：
Hodgetts, SI;Beilharz, MW;Grounds, MD
通讯作者：
Grounds, MD
影响因子：
7.8
作者：
De Angelis, L;Berghella, L;Coletta, M;Lattanzi, L;Zanchi, M;Cusella-De Angelis, MG;Ponzetto, C;Cossu, G
通讯作者：
Cossu, G