The transcriptome of the developing grain: a resource for understanding seed development and the molecular control of the functional and nutritional properties of wheat.
The transcriptome of the developing grain: a resource for understanding seed development and the molecular control of the functional and nutritional properties of wheat.
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DOI：
10.1186/s12864-017-4154-z
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发表时间：
2017-10-11
期刊：
影响因子：
4.4
通讯作者：
Henry RJ
中科院分区：
文献类型：
--
作者：
Rangan P;Furtado A;Henry RJ
小麦是三种主要的谷物之一，已经被驯化来养活人类。小麦籽粒的组成决定了小麦的功能特性，包括碾磨效率、面包制作和营养价值。小麦籽粒发育的转录组分析为了解籽粒发育和品质的分子基础提供了关键的见解。通过RNA-Seq在两个发育阶段（开花后14天和30天，dpa）分析了35个基因型的转录组，这两个发育阶段对应于发育中期（阶段Z75）和几乎成熟的种子（阶段Z 85）。在14 dpa时，大部分转录本与种子主要成分的合成有关，包括贮藏蛋白和淀粉。在30 dpa时，各种各样的基因以低水平表达，其中与种子防御和胁迫耐受性相关的基因占优势。14 dpa和30 dpa阶段之间表达变化的RNA-Seq分析显示，在≤0.01的FDR校正p值截止值下，有26，477个转录物显著差异表达。进行功能注释和基因本体映射，并且KEGG途径映射允许基于生化连接进行分组。结果表明，与果皮相关的光合作用在14 dpa时非常活跃，但在30 dpa时已经停止。最近报道的面粉产量和面包品质的基因被发现影响小麦品质很大程度上是由于在早期种子发育阶段的表达模式。这项研究作为一个资源，提供了一个概述的基因表达过程中小麦籽粒发育的早期（14 dpa）和后期（30 dpa）灌浆阶段用于研究谷物品质和营养价值，并在了解种子生物学。本文的在线版本doi：（10.1186/s12864-017-4154-z）包含补充材料，可供授权用户使用。
Wheat is one of the three major cereals that have been domesticated to feed human populations. The composition of the wheat grain determines the functional properties of wheat including milling efficiency, bread making, and nutritional value. Transcriptome analysis of the developing wheat grain provides key insights into the molecular basis for grain development and quality. The transcriptome of 35 genotypes was analysed by RNA-Seq at two development stages (14 and 30 days-post-anthesis, dpa) corresponding to the mid stage of development (stage Z75) and the almost mature seed (stage Z85). At 14dpa, most of the transcripts were associated with the synthesis of the major seed components including storage proteins and starch. At 30dpa, a diverse range of genes were expressed at low levels with a predominance of genes associated with seed defence and stress tolerance. RNA-Seq analysis of changes in expression between 14dpa and 30dpa stages revealed 26,477 transcripts that were significantly differentially expressed at a FDR corrected p-value cut-off at ≤0.01. Functional annotation and gene ontology mapping was performed and KEGG pathway mapping allowed grouping based upon biochemical linkages. This analysis demonstrated that photosynthesis associated with the pericarp was very active at 14dpa but had ceased by 30dpa. Recently reported genes for flour yield in milling and bread quality were found to influence wheat quality largely due to expression patterns at the earlier seed development stage. This study serves as a resource providing an overview of gene expression during wheat grain development at the early (14dpa) and late (30dpa) grain filling stages for use in studies of grain quality and nutritional value and in understanding seed biology. The online version of this article doi: (10.1186/s12864-017-4154-z) contains supplementary material, which is available to authorized users.
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