Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers.
Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers.
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DOI：
10.1038/s41467-018-03940-3
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发表时间：
2018-04-24
影响因子：
16.6
通讯作者：
Gotoh Y
中科院分区：
文献类型：
--
作者：
Lanjakornsiripan D;Pior BJ;Kawaguchi D;Furutachi S;Tahara T;Katsuyama Y;Suzuki Y;Fukazawa Y;Gotoh Y
非脑顶新皮质星形胶质细胞历来被认为主要由原生质星形胶质细胞组成。本研究表明，小鼠体感觉皮层的星形胶质细胞表现出层特异性形态和分子差异。二维和三维观察显示，不同层的星形胶质细胞具有不同的形态，反映在细胞取向、领土体积和树形上的差异。II/III层星形胶质细胞对突触间隙的成鞘程度大于VI层星形胶质细胞，且上层和深层星形胶质细胞的基因表达存在差异。重要的是，在reeler和Dab1条件敲除小鼠中，星形胶质细胞特性的层特异性差异被消除，其中神经元层受到干扰，这表明神经元层是观察到的新皮层星形胶质细胞形态和分子差异的先决条件。因此，这项研究表明神经元和星形胶质细胞之间存在层特异性相互作用，这可能是它们层特异性功能的基础。几项研究表明，新皮层中的星形胶质细胞比以前认为的更加多样化。在这里，作者描述了星形胶质细胞的形态和分子特征的层特异性差异，这些差异依赖于这些层内的神经元。
Non-pial neocortical astrocytes have historically been thought to comprise largely a nondiverse population of protoplasmic astrocytes. Here we show that astrocytes of the mouse somatosensory cortex manifest layer-specific morphological and molecular differences. Two- and three-dimensional observations revealed that astrocytes in the different layers possess distinct morphologies as reflected by differences in cell orientation, territorial volume, and arborization. The extent of ensheathment of synaptic clefts by astrocytes in layer II/III was greater than that by those in layer VI. Moreover, differences in gene expression were observed between upper-layer and deep-layer astrocytes. Importantly, layer-specific differences in astrocyte properties were abrogated in reeler and Dab1 conditional knockout mice, in which neuronal layers are disturbed, suggesting that neuronal layers are a prerequisite for the observed morphological and molecular differences of neocortical astrocytes. This study thus demonstrates the existence of layer-specific interactions between neurons and astrocytes, which may underlie their layer-specific functions. Several studies have suggested that astrocytes in the neocortex are more diverse than previously thought. Here, the authors describe layer-specific differences in morphology and molecular characteristics of astrocytes that depend on the neurons within those layers.
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影响因子：
25
作者：
John Lin CC;Yu K;Hatcher A;Huang TW;Lee HK;Carlson J;Weston MC;Chen F;Zhang Y;Zhu W;Mohila CA;Ahmed N;Patel AJ;Arenkiel BR;Noebels JL;Creighton CJ;Deneen B
通讯作者：
Deneen B
影响因子：
3.7
作者：
Garcia-Marques, Jorge;Lopez-Mascaraque, Laura
通讯作者：
Lopez-Mascaraque, Laura
DOI：
10.1073/pnas.1320401111
发表时间：
2014-07-22
影响因子：
11.1
作者：
Kang, Wenfei;Balordi, Francesca;Hebert, Jean M.
通讯作者：
Hebert, Jean M.
影响因子：
16.2
作者：
Belgard TG;Marques AC;Oliver PL;Abaan HO;Sirey TM;Hoerder-Suabedissen A;García-Moreno F;Molnár Z;Margulies EH;Ponting CP
通讯作者：
Ponting CP
影响因子：
25
作者：
Madisen L;Zwingman TA;Sunkin SM;Oh SW;Zariwala HA;Gu H;Ng LL;Palmiter RD;Hawrylycz MJ;Jones AR;Lein ES;Zeng H
通讯作者：
Zeng H