A transcriptomic atlas of mouse neocortical layers.
A transcriptomic atlas of mouse neocortical layers.
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小鼠新皮层的转录图。
DOI：
10.1016/j.neuron.2011.06.039
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发表时间：
2011-08-25
期刊：
影响因子：
16.2
通讯作者：
Ponting CP
中科院分区：
文献类型：
--
作者：
Belgard TG;Marques AC;Oliver PL;Abaan HO;Sirey TM;Hoerder-Suabedissen A;García-Moreno F;Molnár Z;Margulies EH;Ponting CP
在哺乳动物的皮质中，神经元和神经胶质形成了一个跨越六层的模式化结构，其复杂的细胞结构排列可能有助于认知。我们对成年（出生后第56天）小鼠体感皮层不同区域（初级和次级）1- 6 b层的转录组进行测序，以了解构成这些层的细胞的编码和非编码基因座的转录水平和功能库。基于机器学习模型（朴素贝叶斯）方法，共有5，835个蛋白质编码基因和66个非编码RNA基因座在各层中差异表达（“模式化”）。层2- 6 b各自与特定的功能和疾病注释相关联，这些注释提供了对其生物学作用的见解。这种新的资源（http：//genserv.anat.ox.ac.uk/layers）通过提供定量表达水平、通过是全基因组的、通过包括新的基因座以及通过鉴定跨层差异表达的候选可变剪接转录物，极大地扩展了当前可用的资源，例如艾伦小鼠脑图谱和微阵列数据集。新皮质层全基因组转录的在线图谱疾病基因和特定层之间的显著，重复的关联跨层广泛的同种型转换LincRNA保守，与相邻的蛋白质编码基因跨层共表达
In the mammalian cortex, neurons and glia form a patterned structure across six layers whose complex cytoarchitectonic arrangement is likely to contribute to cognition. We sequenced transcriptomes from layers 1-6b of different areas (primary and secondary) of the adult (postnatal day 56) mouse somatosensory cortex to understand the transcriptional levels and functional repertoires of coding and noncoding loci for cells constituting these layers. A total of 5,835 protein-coding genes and 66 noncoding RNA loci are differentially expressed (“patterned”) across the layers, on the basis of a machine-learning model (naive Bayes) approach. Layers 2-6b are each associated with specific functional and disease annotations that provide insights into their biological roles. This new resource (http://genserv.anat.ox.ac.uk/layers) greatly extends currently available resources, such as the Allen Mouse Brain Atlas and microarray data sets, by providing quantitative expression levels, by being genome-wide, by including novel loci, and by identifying candidate alternatively spliced transcripts that are differentially expressed across layers. ► Online atlas of genome-wide transcription across neocortical layers ► Significant, replicated associations between disease genes and specific layers ► Widespread isoform switching across layers ► LincRNAs conserved, coexpressed across layers with neighboring protein-coding genes
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影响因子：
14.9
作者：
Blake JA;Bult CJ;Kadin JA;Richardson JE;Eppig JT;Mouse Genome Database Group
通讯作者：
Mouse Genome Database Group
影响因子：
5.1
作者：
Conti, F;Minelli, A;Melone, M
通讯作者：
Melone, M
影响因子：
11.2
作者：
Biskup, Saskia;Moore, Darren J.;Dawson, Valina L.
通讯作者：
Dawson, Valina L.
DOI：
10.1093/database/bay119
发表时间：
2018-11-06
影响因子：
5.8
作者：
Hunt, Sarah E.;McLaren, William;Cunningham, Fiona
通讯作者：
Cunningham, Fiona
影响因子：
56.9
作者：
Gregg, Christopher;Zhang, Jiangwen;Dulac, Catherine
通讯作者：
Dulac, Catherine