Mobile FT mRNA contributes to the systemic florigen signalling in floral induction.

Mobile FT mRNA contributes to the systemic florigen signalling in floral induction.
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移动 FT mRNA 有助于花诱导中的系统成花素信号传导

DOI:
10.1038/srep00073
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发表时间:
2011
期刊:
影响因子:
4.6
通讯作者:
Hong, Yiguo
Hong, Yiguo
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Li, Chunyang;Gu, Mei;Shi, Nongnong;Zhang, Hang;Yang, Xin;Osman, Toba;Liu, Yule;Wang, Huizhong;Vatish, Manu;Jackson, Stephen;Hong, Yiguo

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在诱导光周期条件下,植物在叶子中产生一种被称为“成花素”的信号。成花素通过韧皮部移动到茎顶端分生组织(SAM),在那里它诱导开花。在拟南芥中,FLOWERLOCUS T(FT)蛋白是韧皮部移动信号的一个组成部分。然而,可转运的FT mRNA是否也有助于系统成花素信号传导仍有待阐明。使用非传统的方法,利用病毒诱导的RNA沉默和分生组织排斥病毒感染,我们证明了拟南芥FT mRNA,独立的FT蛋白,可以移动到SAM。非翻译FT mRNA的病毒异位表达促进了SD中短日照(SD)烟草马里兰州猛犸烟草的早期开花。这些数据表明FT mRNA在系统性花信号传导中的可能作用,并且还表明细胞mRNA到SAM的顺式运输和致病RNA的分生组织排斥是两个机械上不同的过程。
In inducing photoperiodic conditions, plants produce a signal dubbed “florigen” in leaves. Florigen moves through the phloem to the shoot apical meristem (SAM) where it induces flowering. In Arabidopsis, the FLOWERING LOCUS T (FT) protein acts as a component of this phloem-mobile signal. However whether the transportable FT mRNA also contributes to systemic florigen signalling remains to be elucidated. Using non-conventional approaches that exploit virus-induced RNA silencing and meristem exclusion of virus infection, we demonstrated that the Arabidopsis FT mRNA, independent of the FT protein, can move into the SAM. Viral ectopic expression of a non-translatable FT mRNA promoted earlier flowering in the short-day (SD) Nicotiana tabacum Maryland Mammoth tobacco in SD. These data suggest a possible role for FT mRNA in systemic floral signalling, and also demonstrate that cis-transportation of cellular mRNA into SAM and meristem exclusion of pathogenic RNAs are two mechanistically distinct processes.
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