Cardiac electrophysiological imaging systems scalable for high-throughput drug testing.
Cardiac electrophysiological imaging systems scalable for high-throughput drug testing.
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DOI：
10.1007/s00424-012-1149-0
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发表时间：
2012-12
影响因子：
4.5
通讯作者：
Bollensdorff, Christian
中科院分区：
文献类型：
--
作者：
Lee, Peter;Wang, Ken;Woods, Christopher E.;Yan, Ping;Kohl, Peter;Ewart, Paul;Loew, Leslie M.;Terrar, Derek A.;Bollensdorff, Christian
关键词：
使用光学方法的多参数电生理测量已经成为心脏研究中的高度重视的标准。大多数公开的光学映射系统是昂贵且复杂的。虽然有些应用需要高成本的组件和复杂的设计，但许多应用可以通过更简单的解决方案来解决。在这里，我们描述了（1）一个基于相机的电压和钙成像系统，使用一个单一的“经济”的电子倍增电荷耦合器件相机，并证明了使用消费者相机成像的心脏钙瞬变的可能性，和（2）基于光电二极管的电压和钙高时间分辨率测量系统，使用单元件光电二极管和光纤。高通量药物测试代表了系统可扩展性特别有吸引力的应用。因此，我们在暴露于已被充分表征且临床相关的钙通道阻滞剂硝苯地平的组织上测试了我们的系统，硝苯地平已被用于治疗心绞痛和高血压。作为实验模型，我们使用了Langendorff灌注的全心脏和薄心室组织切片，这是心脏研究界重新关注的一种制备方法。使用我们简化的系统，我们能够同时监测电压和钙瞬变的显著变化，这是该化合物负性肌力作用的原因。本文的在线版本（doi：10.1007/s 00424 -012-1149-0）包含补充材料，可供授权用户使用。
Multi-parametric electrophysiological measurements using optical methods have become a highly valued standard in cardiac research. Most published optical mapping systems are expensive and complex. Although some applications demand high-cost components and complex designs, many can be tackled with simpler solutions. Here, we describe (1) a camera-based voltage and calcium imaging system using a single ‘economy’ electron-multiplying charge-coupled device camera and demonstrate the possibility of using a consumer camera for imaging calcium transients of the heart, and (2) a photodiode-based voltage and calcium high temporal resolution measurement system using single-element photodiodes and an optical fibre. High-throughput drug testing represents an application where system scalability is particularly attractive. Therefore, we tested our systems on tissue exposed to a well-characterized and clinically relevant calcium channel blocker, nifedipine, which has been used to treat angina and hypertension. As experimental models, we used the Langendorff-perfused whole-heart and thin ventricular tissue slices, a preparation gaining renewed interest by the cardiac research community. Using our simplified systems, we were able to monitor simultaneously the marked changes in the voltage and calcium transients that are responsible for the negative inotropic effect of the compound. The online version of this article (doi:10.1007/s00424-012-1149-0) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Entcheva, E;Kostov, Y;Tung, L
通讯作者：
Tung, L
影响因子：
5.5
作者：
Fedorov, Vadim V.;Lozinsky, Ilya T.;Efimov, Igor R.
通讯作者：
Efimov, Igor R.
DOI：
10.1046/j.1540-8167.2003.02077.x
发表时间：
2003-01-01
影响因子：
2.7
作者：
Kong, W;Walcott, GP;Knisley, SB
通讯作者：
Knisley, SB
影响因子：
3.4
作者：
Hooks, DA;LeGrice, IJ;Smaill, BH
通讯作者：
Smaill, BH
DOI：
10.1016/0167-4889(91)90214-i
发表时间：
1991-01-10
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BOWMASTER, TA;DAVIS, CC;KRAUTHAMER, V
通讯作者：
KRAUTHAMER, V