Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil
Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil
复制标题
利用固菌棉纤维吸附降解原油
DOI：
10.1016/j.ibiod.2013.11.015
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发表时间：
2014-03
影响因子：
4.8
通讯作者：
Xiaoke Hu
中科院分区：
文献类型：
--
作者：
Meng Lin;Yuhua Liu;Weiwei Chen;Hui Wang;Xiaoke Hu
利用富集培养技术，从渤海湾污染沉积物中分离得到两株对原油具有显著降解和分散作用的菌株，16S rRNA基因测序和系统发育分析表明，这两株菌株分别属于弧菌属和不动杆菌属。随后，将细菌细胞固定在棉纤维表面。以棉纤维作为原油吸附剂和细菌固定化的生物载体。其中，海洋细菌为不动杆菌。HC8-3S与棉纤维有较强的结合，可能通过胞外分散剂由不动杆菌分泌而增强。HC8-3S。在pH 5.6 ~ 8.6范围内，浮游菌和固定化菌对原油饱和烃组分均有较高的生物降解率（bbb60 %）。当NaCl浓度达到70 g/L时，浮游菌和固定化菌的降解活性不受显著影响。固定化细菌细胞对原油的生物降解能力增强。固定化细菌细胞对饱和烃的降解效率比浮游细菌细胞提高了约30%。
Using enrichment culture technique, two isolates that brought a significant degradation and dispersion of crude oil were obtained from contaminated sediments of the Bohai Bay, China. 16S rRNA gene sequencing and phylogenetic analysis indicated that the two bacterial strains affiliated with the generaVibrioandAcinetobacter. Subsequently, the bacterial cells were immobilized on the surface of cotton fibers. Cotton fibers were used as crude oil sorbent as well as a biocarrier for bacteria immobilization. Among the two isolates, the marine bacteriaAcinetobactersp. HC8-3S showed a strong binding to the cotton fibers, possibly enhanced through extracellular dispersant excreted byAcinetobactersp. HC8-3S. Both planktonic and immobilized bacteria showed relatively high biodegradation (>60%) of saturated hydrocarbons fraction of crude oil, in the pH range of 5.6–8.6. The degradation activities of planktonic and immobilized bacteria were not affected significantly when the NaCl concentration reached 70 g/L. The immobilized bacterial cells exhibited an enhanced biodegradation of crude oil. The efficiency of saturated hydrocarbons degradation by the immobilized bacterial cells increased about 30% compared to the planktonic bacterial cells.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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