High-Level Expression of Toll-Like Receptors on Dendritic Cells in Adult Patients with Burns on ≥90% of Total Body Surface Area (TBSA).
High-Level Expression of Toll-Like Receptors on Dendritic Cells in Adult Patients with Burns on ≥90% of Total Body Surface Area (TBSA).
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高水平%20表达%20of%20Toll-Like%20受体%20on%20Dendritic%20Cells%20in%20Adult%20Patients%20with%20Burns%20on%20>=%2090%%20of%20Total%20Body%20Surface%20Area%20
DOI：
10.12659/msm.897433
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发表时间：
2016-09-30
期刊：
影响因子：
--
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Zhang X;Li N;Meng Y;Zhang R;Bian J;Yao Y;Li J;Deng X
严重烧伤是一个严重的临床问题，严重烧伤后机体免疫功能紊乱，导致机体免疫功能的进行性抑制。TLR与免疫系统激活相关，但TLR水平对严重烧伤患者循环cDC的影响尚未得到充分评估。本研究纳入了10名在我们机构住院的全身表面积（TBSA）烧伤>90%的患者。我们通过流式细胞术分析烧伤后14天至28天患者循环cDC上的TLR2、TLR4和TLR9表达，根据死亡率，我们还评估了人口统计学、临床结局、器官功能以及炎症和急性期反应。在伤后第一个14天之前，存活和不存活的烧伤患者的TBSA、性别、年龄或手术次数没有差异。与年龄匹配的健康志愿者相比，所有患者循环cDC中的TLR2、TLR4和TLR9水平显著且一致地升高，并且幸存者表现出比非幸存者更高的TLR2和TLR4值。在存活者中，TLR2和TLR4水平在伤后28天高于伤后14天，而TLR9水平差异不显著。伤后28 d死亡组TLR2水平下降，TLR4和TLR9水平无显著性差异。在严重烧伤患者中，循环cDC中的TLR水平在损伤后长达28天内高度活化。cDCs中TLR2的低表达可能是预测严重烧伤患者预后不良的一个潜在指标。
As a serious clinical problem, severe burn injury disturbs the immune system, resulting in progressive suppression of immune response. TLRs are associated with immune system activation, but the effect of TLRs levels on circulating cDCs of severe burn injury patients has not been fully assessed. Ten patients with total body surface area (TBSA) burned >90% admitted to our institution were enrolled in this study. We analyzed TLR2, TLR4, and TLR9 expression on the circulating cDCs by using multicolor flow cytometric analysis in patients at 14 days to 28 days after burn injury according to mortality, and We also assessed Demographics, clinical outcomes, organ function, and inflammatory and acute-phase responses. No difference in TBSA, sex, age, or number of operations before the first 14 days after injury were observed between surviving and non-surviving burn patients. The levels of TLR2, TLR4, and TLR9 in circulating cDCs were significantly and consistently elevated in all patients compared to age-matched healthy volunteers, and survivors exhibited higher TLR2 and TLR4 values than non-survivors. Of the survivors, TLR2 and TLR4 levels were higher at 28 days than at 14 days after injury, while the difference in TLR9 levels was not significant. TLR2 levels of non-survivors at 28 days after injury decreased, and the TLR4 and TLR9 levels showed no significant difference. TLRs levels in circulating cDCs are highly activated in severe burn injury patients up to 28 days after injury. The low expression of TLR2 in cDCs may be useful as a potential marker predicting the poor prognosis of severe burn patients.
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DOI：
10.1186/cc9300
发表时间：
2010
期刊：
Critical care (London, England)
影响因子：
--
作者：
Brusselaers N;Monstrey S;Vogelaers D;Hoste E;Blot S
通讯作者：
Blot S
影响因子：
2.7
作者：
D'Avignon, Laurie C.;Hogan, Brian K.;Wolf, Steven E.
通讯作者：
Wolf, Steven E.
DOI：
10.1097/01.ta.0000238228.67894.d7
发表时间：
2006-09-01
影响因子：
--
作者：
Mohri, Tomoyoshi;Ogura, Hiroshi;Sugimoto, Hisashi
通讯作者：
Sugimoto, Hisashi
DOI：
10.4103/0970-0358.70715
发表时间：
2010-09
期刊：
Indian journal of plastic surgery : official publication of the Association of Plastic Surgeons of India
影响因子：
--
作者：
Haberal M;Sakallioglu Abali AE;Karakayali H
通讯作者：
Karakayali H
影响因子：
8.8
作者：
Paratz, Jennifer D.;Lipman, Jeffrey;Paterson, David L.
通讯作者：
Paterson, David L.