Genetic architecture of a hormonal response to gene knockdown in honey bees.
Genetic architecture of a hormonal response to gene knockdown in honey bees.
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DOI：
10.1093/jhered/esu086
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Amdam GV
中科院分区：
文献类型：
--
作者：
Ihle KE;Rueppell O;Huang ZY;Wang Y;Fondrk MK;Page RE Jr;Amdam GV
内分泌信号的变化被认为是社会生活史进化和调节的基础，但内分泌信号的遗传结构仍然知之甚少。在蜜蜂（Apis mellifera）中发现了一组受激素影响的社会特征的极好例子：幼代激素（JH）和蛋黄前体蛋白卵黄原蛋白（Vg）之间的动态相互抑制关系调节了工蜂的行为成熟和觅食。其他一些性状与这些行为表型共分离，构成花粉囤积综合征（PHS）是描述最好的动物行为综合征之一。JH对Vg反应性的基因型差异是PHS的潜在机制基础。在这里，我们通过RNA干扰降低了Vg的表达，这是由2个不同的蜜蜂品系对Vg降低的反应性和测量的JH反应和卵巢大小的回交后代得到的，这代表了小灵通的另一个关键方面。基于限制性内切位点相关DNA标记测序的遗传定位鉴定出卵巢大小和JH响应性的QTL。我们在许多QTL附近证实了这两个性状的遗传效应，这些QTL对小灵通的各种性状有影响。因此，我们的研究结果支持在遗传水平上内分泌控制复杂性状的作用。此外，这是社会性昆虫对基因敲除的激素反应基因图谱的第一个例子，有助于完善对复杂行为和生理的遗传理解，这可能是行为控制的基础。
Variation in endocrine signaling is proposed to underlie the evolution and regulation of social life histories, but the genetic architecture of endocrine signaling is still poorly understood. An excellent example of a hormonally influenced set of social traits is found in the honey bee (Apis mellifera): a dynamic and mutually suppressive relationship between juvenile hormone (JH) and the yolk precursor protein vitellogenin (Vg) regulates behavioral maturation and foraging of workers. Several other traits cosegregate with these behavioral phenotypes, comprising the pollen hoarding syndrome (PHS) one of the best-described animal behavioral syndromes. Genotype differences in responsiveness of JH to Vg are a potential mechanistic basis for the PHS. Here, we reduced Vg expression via RNA interference in progeny from a backcross between 2 selected lines of honey bees that differ in JH responsiveness to Vg reduction and measured JH response and ovary size, which represents another key aspect of the PHS. Genetic mapping based on restriction site-associated DNA tag sequencing identified suggestive quantitative trait loci (QTL) for ovary size and JH responsiveness. We confirmed genetic effects on both traits near many QTL that had been identified previously for their effect on various PHS traits. Thus, our results support a role for endocrine control of complex traits at a genetic level. Furthermore, this first example of a genetic map of a hormonal response to gene knockdown in a social insect helps to refine the genetic understanding of complex behaviors and the physiology that may underlie behavioral control in general.
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影响因子：
3.7
作者：
Dallacqua RP;Bitondi MM
通讯作者：
Bitondi MM
影响因子：
3.7
作者：
Baird NA;Etter PD;Atwood TS;Currey MC;Shiver AL;Lewis ZA;Selker EU;Cresko WA;Johnson EA
通讯作者：
Johnson EA
DOI：
10.1534/g3.112.004093
发表时间：
2012-11
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Aranjuez G;Kudlaty E;Longworth MS;McDonald JA
通讯作者：
McDonald JA
DOI：
10.1016/s0169-328x(01)00325-4
发表时间：
2002-03-28
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Kucharski, R;Maleszka, R
通讯作者：
Maleszka, R
影响因子：
2.3
作者：
CALDERONE, NW;PAGE, RE
通讯作者：
PAGE, RE