Dimorphic ovary differentiation in honeybee (Apis mellifera) larvae involves caste-specific expression of homologs of ark and buffy cell death genes.
Dimorphic ovary differentiation in honeybee (Apis mellifera) larvae involves caste-specific expression of homologs of ark and buffy cell death genes.
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DOI：
10.1371/journal.pone.0098088
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bitondi MM
中科院分区：
文献类型：
--
作者：
Dallacqua RP;Bitondi MM
卵巢中重复结构单位(卵巢)数量的确定是形成群居昆虫种姓多面性的关键事件之一。在早期胚胎后发育中，蜜蜂幼虫有一对卵巢，每个卵巢由近200个卵巢原基组成。虽然实际上所有这些卵巢小管都在女王型幼虫中继续发育，但它们在工蚁型幼虫中经历了大规模的程序性细胞死亡(PCD)。为了深入了解这一种级分化的基本过程的分子基础，我们利用定量聚合酶链式反应(QPCR)和荧光原位杂交(FISH)技术，研究了Amark和Ambuy基因在两种蜂种5龄幼虫卵巢中的表达。这两个基因分别是方舟和巴菲果蝇黑腹果蝇基因的同源物，它们分别参与激活和抑制PCD。在此时间窗中发现了特定种型的表达模式，该窗口定义了卵巢小管的数量。当工人卵巢吸收加强时，AMAK转录水平升高，但在女王卵巢中保持在较低水平。转录本主要定位于所有工蜂卵巢小管的顶端，但仅在少数蜂后卵巢小管中出现，因此强烈提示在工蜂幼虫体内存在大量卵巢小管细胞死亡的调节功能。Ambuy主要表达于覆盖每个卵巢小管的腹膜鞘细胞。在蜂王的卵巢发育中，Ambuy转录本的水平比工蚁提前增加。与对细胞死亡的保护作用一致，Ambuy转录本在几乎所有的女王卵巢小管中都有定位，但只在少数工作卵巢小管中定位。这些结果表明，进化保守的细胞死亡基因的表达与导致群居昆虫种姓特异性卵巢分化的形态事件之间存在功能关系。
The establishment of the number of repeated structural units, the ovarioles, in the ovaries is one of the critical events that shape caste polyphenism in social insects. In early postembryonic development, honeybee (Apis mellifera) larvae have a pair of ovaries, each one consisting of almost two hundred ovariole primordia. While practically all these ovarioles continue developing in queen-destined larvae, they undergo massive programmed cell death (PCD) in worker-destined larvae. So as to gain insight into the molecular basis of this fundamental process in caste differentiation we used quantitative PCR (qPCR) and fluorescent in situ hybridization (FISH) to investigate the expression of the Amark and Ambuffy genes in the ovaries of the two honeybee castes throughout the fifth larval instar. These are the homologs of ark and buffy Drosophila melanogaster genes, respectively, involved in activating and inhibiting PCD. Caste-specific expression patterns were found during this time-window defining ovariole number. Amark transcript levels were increased when ovariole resorption was intensified in workers, but remained at low levels in queen ovaries. The transcripts were mainly localized at the apical end of all the worker ovarioles, but appeared in only a few queen ovarioles, thus strongly suggesting a function in mediating massive ovariolar cell death in worker larvae. Ambuffy was mainly expressed in the peritoneal sheath cells covering each ovariole. The levels of Ambuffy transcripts increased earlier in the developing ovaries of queens than in workers. Consistent with a protective role against cell death, Ambuffy transcripts were localized in practically all queen ovarioles, but only in few worker ovarioles. The results are indicative of a functional relationship between the expression of evolutionary conserved cell death genes and the morphological events leading to caste-specific ovary differentiation in a social insect.
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高度社会性蜜蜂意大利蜜蜂种姓分化的分子决定因素。
影响因子：
--
作者：
Barchuk AR;Cristino AS;Kucharski R;Costa LF;Simões ZL;Maleszka R
通讯作者：
Maleszka R
DOI：
10.1073/pnas.97.2.662
发表时间：
2000-01-18
影响因子：
11.1
作者：
Igaki, T;Kanuka, H;Miura, M
通讯作者：
Miura, M
影响因子：
3.6
作者：
Capella, ICS;Hartfelder, K
通讯作者：
Hartfelder, K
影响因子：
0.8
作者：
Hartfelder, K;Steinbruck, G
通讯作者：
Steinbruck, G
影响因子：
2.2
作者：
de Azevedo, Sergio Vicente;Hartfelder, Klaus
通讯作者：
Hartfelder, Klaus