Physiological properties of cholinergic and non-cholinergic magnocellular neurons in acute slices from adult mouse nucleus basalis.
Physiological properties of cholinergic and non-cholinergic magnocellular neurons in acute slices from adult mouse nucleus basalis.
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DOI：
10.1371/journal.pone.0011046
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发表时间：
2010-06-10
期刊：
影响因子：
3.7
通讯作者：
Waters J
中科院分区：
文献类型：
--
作者：
Hedrick T;Waters J
基底前脑是一系列的核团，为前脑的大部分提供胆碱能输入。这些核团最后面的基底核为新皮质提供胆碱能驱动，参与觉醒和注意。前脑前基底核，包括内侧隔核、Broca斜角带和无名质神经元的生理特性已有描述。相比之下，基底前脑最后部的基底核的神经元的生理特性尚不清楚。在这里，我们研究了成年小鼠基底核神经元的生理特性。我们从p42-54小鼠的切片中获得了大细胞神经元的细胞附着和全细胞记录，并比较了胆碱能神经元和非胆碱能神经元，通过抗胆碱乙酰转移酶免疫细胞化学进行了回顾性区分。大多数(70-80%)胆碱能和非胆碱能神经元在静息状态下保持沉默。自发兴奋的胆碱能神经元和非胆碱能神经元分别在3 Hz和0.3~13.4 Hz出现不规则峰电位。胆碱能神经元的动作电位较非胆碱能神经元更窄、更宽(波幅±3.4和75±2 mV；半宽0.52±0.04和0.33±0.02ms)。胆碱能神经元表现出比非胆碱能神经元更明显的慢后超极化(13.3±2.2 mV和3.6±0.5 mV)，并且不能在紧张性电流注射时出现高频峰(最大频率为∼2 0 Hz和1 2 0 Hz)。结果表明，基底核神经元与基底前脑前部神经元具有相似的生理特性。此外，基底核的胆碱能神经元和非胆碱能神经元可以通过它们对注入电流的反应来区分。据我们所知，这是第一次描述了基底前脑复合体后部胆碱能和非胆碱能神经元的生理特性，也是第一次对小鼠基底前脑神经元的研究。
The basal forebrain is a series of nuclei that provides cholinergic input to much of the forebrain. The most posterior of these nuclei, nucleus basalis, provides cholinergic drive to neocortex and is involved in arousal and attention. The physiological properties of neurons in anterior basal forebrain nuclei, including medial septum, the diagonal band of Broca and substantia innominata, have been described previously. In contrast the physiological properties of neurons in nucleus basalis, the most posterior nucleus of the basal forebrain, are unknown. Here we investigate the physiological properties of neurons in adult mouse nucleus basalis. We obtained cell-attached and whole-cell recordings from magnocellular neurons in slices from P42-54 mice and compared cholinergic and non-cholinergic neurons, distinguished retrospectively by anti-choline acetyltransferase immunocytochemistry. The majority (70–80%) of cholinergic and non-cholinergic neurons were silent at rest. Spontaneously active cholinergic and non-cholinergic neurons exhibited irregular spiking at 3 Hz and at 0.3 to 13.4 Hz, respectively. Cholinergic neurons had smaller, broader action potentials than non-cholinergic neurons (amplitudes 64±3.4 and 75±2 mV; half widths 0.52±0.04 and 0.33±0.02 ms). Cholinergic neurons displayed a more pronounced slow after-hyperpolarization than non-cholinergic neurons (13.3±2.2 and 3.6±0.5 mV) and were unable to spike at high frequencies during tonic current injection (maximum frequencies of ∼20 Hz and >120 Hz). Our results indicate that neurons in nucleus basalis share similar physiological properties with neurons in anterior regions of the basal forebrain. Furthermore, cholinergic and non-cholinergic neurons in nucleus basalis can be distinguished by their responses to injected current. To our knowledge, this is the first description of the physiological properties of cholinergic and non-cholinergic neurons in the posterior aspects of the basal forebrain complex and the first study of basal forebrain neurons from the mouse.
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