Significant structural but not physiological changes in cortical neurons of 12-month-old Tg2576 mice.
Significant structural but not physiological changes in cortical neurons of 12-month-old Tg2576 mice.
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12 个月大的 Tg2576 小鼠的皮质神经元发生显着的结构变化，但没有生理变化。
DOI：
10.1016/j.nbd.2008.07.014
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发表时间：
2008-11
影响因子：
6.1
通讯作者：
Luebke JI
中科院分区：
文献类型：
--
作者：
Rocher AB;Kinson MS;Luebke JI
β-淀粉样蛋白（Aβ）在阿尔茨海默病的病因学中起关键作用。暴露于Aβ的锥体细胞树突表现出显著的结构改变，包括树突棘密度降低。为了确定这种结构改变是否会导致电生理学变化，使用生物胞素填充的全细胞膜片钳记录来评估从12个月大的Tg 2576淀粉样前体蛋白（APP）突变体与野生型（Wt）小鼠制备的额叶皮层切片中第3层锥体细胞的电生理学和形态学特性。Tg 2576细胞表现出显著增加的树突长度和体积和减少的棘密度，而棘的总数与Wt没有差异。Tg 2576和Wt细胞在被动膜、动作电位放电或神经元自发兴奋性突触后电流特性方面没有差异。因此，在年轻的Tg 2576小鼠中突变的APP的过表达导致神经元形态学性质的显著变化，其不具有容易明显的功能后果。
Amyloid-beta (Aβ) plays a key role in the etiology of Alzheimer’s disease. Pyramidal cell dendrites exposed to Aβ exhibit dramatic structural alterations, including reduced dendritic spine densities. To determine whether such structural alterations lead to electrophysiological changes, whole-cell patch clamp recordings with biocytin filling were used to assess both the electrophysiological and morphological properties of layer 3 pyramidal cells in frontal cortical slices prepared from 12-month-old Tg2576 amyloid precursor protein (APP) mutant vs. wild-type (Wt) mice. Tg2576 cells exhibited significantly increased dendritic lengths and volumes and decreased spine densities, while the total number of spines was not different from Wt. Tg2576 and Wt cells did not differ with regard to passive membrane, action potential firing or glutamatergic spontaneous excitatory postsynaptic current properties. Thus, overexpression of mutated APP in young Tg2576 mice leads to significant changes in neuronal morphological properties which do not have readily apparent functional consequences.
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影响因子：
16.2
作者：
Kamenetz, F;Tomita, T;Malinow, R
通讯作者：
Malinow, R
影响因子：
2.9
作者：
King, DL;Arendash, GW
通讯作者：
Arendash, GW
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2.9
作者：
Giacchino, J;Criado, JR;Henriksen, S
通讯作者：
Henriksen, S
影响因子：
6.1
作者：
Almeida, CG;Tampellini, D;Gouras, GK
通讯作者：
Gouras, GK
影响因子：
64.8
作者：
Mainen, ZF;Sejnowski, TJ
通讯作者：
Sejnowski, TJ