Bioelectrocatalysis based on direct electron transfer of fungal pyrroloquinoline quinone-dependent dehydrogenase lacking the cytochrome domain
Bioelectrocatalysis based on direct electron transfer of fungal pyrroloquinoline quinone-dependent dehydrogenase lacking the cytochrome domain
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基于缺乏细胞色素结构域的真菌吡咯喹啉醌依赖性脱氢酶直接电子转移的生物电催化
DOI：
10.1016/j.electacta.2020.136982
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发表时间：
2020
影响因子：
6.6
通讯作者：
Nakamura Nobuhumi
中科院分区：
文献类型：
--
作者：
Takeda Kouta;Kusuoka Ryo;Birrell James A.;Yoshida Makoto;Igarashi Kiyohiko;Nakamura Nobuhumi
氧化还原酶和电极之间的直接电子转移（DET）对于理解氧化还原蛋白的基本特征以及开发无介体的生物电子器件至关重要。吡咯并喹啉醌（PQQ）依赖的生物电催化在生物传感器和生物燃料电池方面都有很好的应用前景。然而，有限数量的研究报告了酶和电极中PQQ之间的DET。在这里，PQQ结构域（只含有PQQ辅因子）的真菌PQQ依赖性吡喃糖脱氢酶从灰盖鬼伞，研究了DET与各种电极。一个2-巯基乙醇自组装单层（SAM）涂层的多晶金电极被发现给优秀的DET的PQQ域，导致10倍的催化电流密度相比，裸玻碳。用27 MHz石英晶体微天平（QCM）测得PQQ结构域的固定量为8.2 ± 0.4 pmol/cm 2，表明在SAM修饰的金表面上形成了近似的蛋白单层。为了提高电流密度，在多晶金电极上修饰金纳米颗粒（AuNPs）。重要的是，在优化的条件下，实现了1.6 mA/cm 2的高催化电流密度的氧化f-岩藻糖。总之，这些结果证明了在真菌PQQ依赖性脱氢酶的活性位点中高效的DET至PQQ。因此，我们的PQQ结构域/SAM/AuNPs涂层多晶金电极可以作为一个精致的敏感的生物传感器和高活性的糖氧化阳极的生物燃料电池。
Direct electron transfer (DET) between oxidoreductases and electrodes is crucial for understanding the fundamental features of redox proteins as well as developing mediator-free bioelectronics devices. Bioelectrocatalysis with pyrroloquinoline quinone (PQQ)-dependent dehydrogenases is promising for both biosensors and biofuel cells. However, a limited number of studies have reported DET between PQQ in the enzyme and the electrode. Here, the PQQ domain (containing only the PQQ cofactor) of the fungal PQQ-dependent pyranose dehydrogenase fromCoprinopsis cinerea, was studied in DET with various electrodes. A 2-mercaptoethanol self-assembled monolayer (SAM)-coated polycrystalline gold electrode was found to give excellent DET for the PQQ domain, resulting in 10-times the catalytic current density compared to bare glassy carbon. The amount of immobilized PQQ domain was determined to be 8.2 ± 0.4 pmol/cm2by using a 27 MHz quartz-crystal microbalance (QCM), suggesting an approximate protein monolayer formation on the SAM modified gold surface. To improve current density, gold nanoparticles (AuNPs) were modified on top of polycrystalline gold electrodes. Importantly, high catalytic current densities of 1.6 mA/cm2for the oxidation ofl-fucose were achieved under optimized conditions. Together, these results demonstrate highly efficient DET to PQQ in the active site of the fungal PQQ-dependent dehydrogenase. Thus, our PQQ-domain/SAM/AuNPs coated polycrystalline gold electrode could serve as an exquisitely sensitive biosensor and a highly active sugar oxidizing anode for biofuel cells.
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pH 依赖性电子转移反应和醌血红素蛋白吡喃糖脱氢酶的直接生物电催化。
影响因子：
3.1
作者：
K. Takeda;H. Matsumura;T. Ishida;Makoto Yoshida;K. Igarashi;M. Samejima;H. Ohno;N. Nakamura
通讯作者：
N. Nakamura
影响因子：
4.5
作者：
IKEDA, T;KOBAYASHI, D;NIKI, K
通讯作者：
NIKI, K
影响因子：
3.9
作者：
H. Toyama;F. Mathews;F. Mathews;O. Adachi;O. Adachi;K. Matsushita;K. Matsushita
通讯作者：
H. Toyama;F. Mathews;F. Mathews;O. Adachi;O. Adachi;K. Matsushita;K. Matsushita
DOI：
10.3390/s18051319
发表时间：
2018-04-24
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Bollella P;Gorton L;Antiochia R
通讯作者：
Antiochia R
Aquifex aeolicus 氢化酶修饰的金纳米颗粒电极上的氢生物电氧化：在氢/氧酶生物燃料电池中的应用。
影响因子：
5
作者：
Karen Monsalve;M. Roger;C. Gutiérrez;M. Ilbert;S. Nitsche;D. Byrne;V. Marchi;E. Lojou
通讯作者：
E. Lojou