Degradable, thermo-sensitive poly(N-isopropyl acrylamide)-based scaffolds with controlled porosity for tissue engineering applications.
Degradable, thermo-sensitive poly(N-isopropyl acrylamide)-based scaffolds with controlled porosity for tissue engineering applications.
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DOI：
10.1021/bm100521x
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发表时间：
2010-10-11
影响因子：
6.2
通讯作者：
Ratner, Buddy D.
中科院分区：
文献类型：
--
作者：
Galperin, Anna;Long, Thomas J.;Ratner, Buddy D.
我们已经开发了一种具有可降解性和可控孔隙率的温敏性聚（N-异丙基丙烯酰胺）基支架。以N-异丙基丙烯酰胺、2-亚甲基-1，3-二氧杂环庚烷和聚己内酯二甲基丙烯酸酯为单体，通过光共聚合制备了可生物降解的聚N-异丙基丙烯酰胺水凝胶。探讨了水凝胶的相变温度、溶胀和粘弹性能以及在25 ° C和37°C下的水解降解性。应用球形模板技术制备了具有可控孔径和高度互连多孔结构的水凝胶支架。评估了支架孔径随温度的变化，并且如预期的，当温度升高至37°C时，孔的直径减小。3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）试验结果表明，支架及其降解产物对NIH 3 T3细胞均无细胞毒性。将具有55±5 μm孔径的支架装载有NIH 3 T3细胞，然后升温至37 ℃，将细胞截留在直径约39 μm的孔中，我们发现该尺寸范围对于血管生成和生物整合是最佳的。培养7天后，细胞表现出均匀的浸润和细长的形态。由于可控的单分散孔径，高度互连的架构，完全可降解的化学和热响应特性，这里开发的基于聚NIPAM的支架在组织工程中的应用是有吸引力的。
We have developed a thermoresponsive poly(N-isopropyl acrylamide)-based scaffold with degradability and controlled porosity. Biodegradable poly(N-isopropyl acrylamide) hydrogels were synthesized by photo-copolymerization of N-isopropylacrylamide with 2-methylene-1,3-dioxepane and polycaprolactone dimethacrylate. The hydrogels’ phase transition temperature, swelling and viscoelastic properties, as well as hydrolytic degradability at 25 and 37°C, were explored. A sphere-templating technique was applied to fabricate hydrogel scaffolds with controllable pore size and a highly interconnected porous structure. The scaffold pore diameter change as a function of temperature was evaluated and, as expected, pores decreased in diameter when the temperature was raised to 37°C. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test results suggested neither the scaffolds nor their degradation products were cytotoxic to NIH3T3 cells. Scaffolds with 55±5 μm pore diameter were loaded with NIH3T3 cells and then were warmed to 37°C entrapping cells in pores approximately 39 μm in diameter, a size range we have found to be optimal for angiogenesis and biointegration. Cells showed uniform infiltration and an elongated morphology after 7 days of culture. Due to the controlled monodisperse pore diameter, highly interconnected architecture, fully degradable chemistry and thermoresponsive properties, the polyNIPAM-based scaffolds developed here are attractive for applications in tissue engineering.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
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作者：
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通讯作者：
Ratner, Buddy D.
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通讯作者：
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影响因子：
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作者：
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